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	Online acid barrage stacking anti-salt injection for capillary electrophoresis of 9-fluorenylmethylchloroformate-derivatized amino acids in high ionic strength solutions by uv detection
	Han, Yanli; Zuo, Min; Qi, Li; Liu, Kun; Mao, Lanqun; Chen, Yi
刊名	Electrophoresis
	2006-11-01
卷号	27期号:21页码:4240-4248
关键词	Acid barrage stacking Anti-salt injection Ce 9-fluorenylmethyl chloroformate-amino acids Physiological samples
ISSN号	0173-0835
DOI	10.1002/elps.200600149
通讯作者	Chen, yi(chenyi@iccas.ac.cn)
英文摘要	An acid barrage stacking (abs) method has been shown to be feasible for online antisalt injection in ce of 9-fluorenylmethyl chloroformate (fmoc)-labeled amino acids (aas) detected by common uv absorption. the operation was performed on normal polar ce by sucking in an extra plug of acid following a sample zone, serving as a selective acid barrage to block the backward migration of weak anionic analytes due to a sudden mobility reduction via acid-base reaction which does not affect strong co-ions such as cl- to penetrate the barrage freely. by ce-uv of fmoc-aas in various nacl solutions, the effectiveness of abs was firmly validated, able to stand up to 500 mm nacl and to stack analytes by 10(3)-fold calculated from the uv detection limits, that is 0.01 mu m for abs and 10 mu m for non-stacking injection. the method was also validated by determining trace glu and asp in real samples of rat brain microdialysate, rat serum and human saliva. the intraday rsds were 0.33-4.9% for migration time and 1.8-9.6% for peak area. the recoveries measured by spiking technique were 82-115% for glu and 86-116% for asp. working equations were obtained by plotting peak height vs. concentration at 0.1-50 mu m, with correlation coefficients of > 0.999. the contents of glu and asp were thus found at 0.26-0.83 mu m and 0.24-0.64 mu m respectively, in rat brain microdialyste; 37-40 mu m and 8.4-10 mu m, respectively, in rat serum; and 3.5-5.8 mu m and 1.0-4.1 mu m, respectively in human saliva. they were consistent with the data from other methods.
WOS关键词	DYNAMIC PH JUNCTION ; TRANSIENT ISOTACHOPHORETIC PRECONCENTRATION ; SOLID-PHASE EXTRACTION ; ZONE-ELECTROPHORESIS ; BACKGROUND ELECTROLYTE ; ARTIFICIAL SEAWATER ; ANALYTES ; SAMPLES ; ANIONS ; SERUM
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
语种	英语
出版者	WILEY-V C H VERLAG GMBH
WOS记录号	WOS:000242222500015
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2379912
专题	中国科学院大学
通讯作者	Chen, Yi
作者单位	1.Chinese Acad Sci, Inst Chem, Lab Analyt Chem Life Sci, Beijing Natl Lab Mol Sci, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Han, Yanli,Zuo, Min,Qi, Li,et al. Online acid barrage stacking anti-salt injection for capillary electrophoresis of 9-fluorenylmethylchloroformate-derivatized amino acids in high ionic strength solutions by uv detection[J]. Electrophoresis,2006,27(21):4240-4248.
APA	Han, Yanli,Zuo, Min,Qi, Li,Liu, Kun,Mao, Lanqun,&Chen, Yi.(2006).Online acid barrage stacking anti-salt injection for capillary electrophoresis of 9-fluorenylmethylchloroformate-derivatized amino acids in high ionic strength solutions by uv detection.Electrophoresis,27(21),4240-4248.
MLA	Han, Yanli,et al."Online acid barrage stacking anti-salt injection for capillary electrophoresis of 9-fluorenylmethylchloroformate-derivatized amino acids in high ionic strength solutions by uv detection".Electrophoresis 27.21(2006):4240-4248.

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