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	The gene ncgl2918 encodes a novel maleylpyruvate isomerase that needs mycothiol as cofactor and links mycothiol biosynthesis and gentisate assimilation in corynebacterium glutamicum
	Feng, J; Che, YS; Milse, J; Yin, YJ; Liu, L; Ruckert, C; Shen, XH; Qi, SW; Kalinowski, J; Liu, SJ
刊名	Journal of biological chemistry
	2006-04-21
卷号	281期号:16页码:10778-10785
ISSN号	0021-9258
DOI	10.1074/jbc.m513192200
通讯作者	Liu, sj()
英文摘要	Data mining of the corynebacterium glutamicum genome identified 4 genes analogous to the msha, mshb, mshc, and mshd genes that are involved in biosynthesis of mycothiol in mycobacterium tuberculosis and mycobacterium smegmatis. individual deletion of these genes was carried out in this study. mutants mshc(-) and mshd(-) lost the ability to produce mycothiol, but mutant mshb(-) produced mycothiol as the wild type did. the phenotypes of mutants mshc(-) and mshd(-) were the same as the wild type when grown in lb or bhis media, but mutants mshc(-) and mshd(-) were not able to grow in mineral medium with gentisate or 3-hydroxybenzoate as carbon sources. c. glutamicum assimilated gentisate and 3-hydroxybenzoate via a glutathione-independent gentisate pathway. in this study it was found that the maleylpyruvate isomerase, which catalyzes the conversion of maleylpyruvate into fumarylpyruvate in the glutathione-independent gentisate pathway, needed mycothiol as a cofactor. this mycothiol-dependent maleylpyruvate isomerase gene (ncgl2918) was cloned, actively expressed, and purified from escherichia coli. the purified mycothiol-dependent isomerase is a monomer of 34 kda. the apparent k-m and v-max values for maleylpyruvate were determined to be 148.4 +/- 11.9 mu m and 1520 +/- 57.4 mu mol/min/mg, respectively (mycothiol concentration, 2.5 mu m). previous studies had shown that mycothiol played roles in detoxification of oxidative chemicals and antibiotics in streptomycetes and mycobacteria. to our knowledge, this is the first demonstration that mycothiol is essential for growth of c. glutamicum with gentisate or 3-hydroxybenzoate as carbon sources and the first characterization of a mycothiol-dependent maleylpyruvate isomerase.
WOS关键词	MYCOBACTERIUM-SMEGMATIS MUTANTS ; COMPLETE GENOME SEQUENCE ; STRUCTURAL-ANALYSIS ; OVOTHIOL-A ; STRAIN U2 ; PATHWAY ; IDENTIFICATION ; THIOL ; DEGRADATION ; METABOLISM
WOS研究方向	Biochemistry & Molecular Biology
WOS类目	Biochemistry & Molecular Biology
语种	英语
出版者	AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
WOS记录号	WOS:000236822200017
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2379868
专题	中国科学院大学
通讯作者	Liu, SJ
作者单位	1.Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing 100080, Peoples R China 2.Univ Bielefeld, Inst Genomforsch, D-33615 Bielefeld, Germany 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Feng, J,Che, YS,Milse, J,et al. The gene ncgl2918 encodes a novel maleylpyruvate isomerase that needs mycothiol as cofactor and links mycothiol biosynthesis and gentisate assimilation in corynebacterium glutamicum[J]. Journal of biological chemistry,2006,281(16):10778-10785.
APA	Feng, J.,Che, YS.,Milse, J.,Yin, YJ.,Liu, L.,...&Liu, SJ.(2006).The gene ncgl2918 encodes a novel maleylpyruvate isomerase that needs mycothiol as cofactor and links mycothiol biosynthesis and gentisate assimilation in corynebacterium glutamicum.Journal of biological chemistry,281(16),10778-10785.
MLA	Feng, J,et al."The gene ncgl2918 encodes a novel maleylpyruvate isomerase that needs mycothiol as cofactor and links mycothiol biosynthesis and gentisate assimilation in corynebacterium glutamicum".Journal of biological chemistry 281.16(2006):10778-10785.

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