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	Identification of genes induced during medicago sativa nodule development by using the cdna-aflp technique
	Xie Rong; He Zhishui; Liu Fanghua; Zou Huasong; Zhu Jiabi; Yu Guanqiao
刊名	Chinese science bulletin
	2006-09-01
卷号	51期号:17页码:2087-2094
关键词	Cdna-amplified fragment length polymorphism Medicago sativa Nodule development Symbiotic nitrogen fixation Sinorhizobium meliloti
ISSN号	1001-6538
DOI	10.1007/s11434-006-2093-1
通讯作者	Yu guanqiao(gqyu@iris.sipp.ac.cn)
英文摘要	A variety of genes involved in nodule development and function were induced during symbiotic root nodule development in alfalfa. we identified those genes by comparing the cdna-amplified fragment length polymorphism (cdna-aflp) patterns of infected nodules and uninfected roots. seventy-one transcript-derived fragments (tdfs) were isolated from about 3000 tdfs, which are enhanced or specifically expressed in nodule. the differential expression patterns of 61 genes were confirmed by reverse northern hybridization or northern hybridization analyses. among these, 31 exhibited significant similarities to characterized database entries. these results suggest that the genes corresponding to the 31 tdfs may play important roles in signal transduction, gene expression and regulation, substance transportation or auto-regulation of nodulation during nodule development. the northern hybridization analysis also indicates that the gene corresponding to tdf rx89 was not expressed in the nodule elicited by baca mutant, presumably it is specially induced at the stage of nodule development when bacteroids begin to be differentiated.
WOS关键词	RECEPTOR KINASE GENE ; EXPRESSION PROFILES ; NITROGEN-FIXATION ; ROOT DEVELOPMENT ; LOTUS-JAPONICUS ; MODEL LEGUME ; TRUNCATULA ; PLANT ; NODULATION ; MUTANTS
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
语种	英语
出版者	SCIENCE CHINA PRESS
WOS记录号	WOS:000241849200009
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2379778
专题	中国科学院大学
通讯作者	Yu Guanqiao
作者单位	1.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Natl Lab Plant Mol Genet, Shanghai 200032, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Xie Rong,He Zhishui,Liu Fanghua,et al. Identification of genes induced during medicago sativa nodule development by using the cdna-aflp technique[J]. Chinese science bulletin,2006,51(17):2087-2094.
APA	Xie Rong,He Zhishui,Liu Fanghua,Zou Huasong,Zhu Jiabi,&Yu Guanqiao.(2006).Identification of genes induced during medicago sativa nodule development by using the cdna-aflp technique.Chinese science bulletin,51(17),2087-2094.
MLA	Xie Rong,et al."Identification of genes induced during medicago sativa nodule development by using the cdna-aflp technique".Chinese science bulletin 51.17(2006):2087-2094.

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