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	An l-glutamate amperometric biosensor based on redox polymer wired horseradish peroxidase and glutamate oxidase
	Li Huaqing; Guo Zonghui; Liu Chunxiu; Jiang Liying; Cui Dafu; Liu Wenyong; Huang Yong; Cai Xinxia
刊名	Chinese journal of analytical chemistry
	2006-09-01
卷号	34页码:S1-s4
ISSN号	0253-3820
通讯作者	Cai xinxia()
英文摘要	A two-electrode configuration has been fabricated by depositing gold film on a polyethylene terephthalate (pet) substrate. the integrated reference electrode was obtained by screen-printing commercial silver and silver chloride paste on the gold electrode. the working electrode was modified by drop-coating a redox polymer formed of poly (4-vinylpyrodine) complexed with osmium (2, 2-bipyrridine)(2). the bienzyme, horseradish peroxidase (hrp and glutamate oxidase (glox), were immobilized in the redox matrix by the (poly(ethylene glycol) diglycidyl ether(pegdge) crosslinker. the optimized biosensor were characterized by a sensitivity of 37.5 ma(mol/l)(-1) cm(-2), a detection limit of 1.0 x 10(-6) mol/l, a response time of less than 16 s, and a correlation coefficient during a concentration ranged from 1.0 x 10(-6) mol/l to 1.0 x 10(-4) mol/l is 0.9998 (n = 9). the biosensor exhibits excellent reproducibility and stability, retaining more than 90% of its original activity over a period of one month. the simple fabrication of the biosensor can be used to determinate l-glutamate rapidly in food industry and clinical diagnose.
WOS关键词	ENZYME ELECTRODE ; DEHYDROGENASE
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
语种	英语
出版者	SCIENCE CHINA PRESS
WOS记录号	WOS:000244539600001
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2379712
专题	中国科学院大学
通讯作者	Cai Xinxia
作者单位	1.Chinese Acad Sci, Inst Elect, State Key Lab Transducer Technol, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100080, Peoples R China 3.Chinese Acad Sci, Inst Chem, Joint Lab Polymer Sci & Mat, State Key Lab Polymer Phys & Chem, Beijing 100080, Peoples R China
推荐引用方式 GB/T 7714	Li Huaqing,Guo Zonghui,Liu Chunxiu,et al. An l-glutamate amperometric biosensor based on redox polymer wired horseradish peroxidase and glutamate oxidase[J]. Chinese journal of analytical chemistry,2006,34:S1-s4.
APA	Li Huaqing.,Guo Zonghui.,Liu Chunxiu.,Jiang Liying.,Cui Dafu.,...&Cai Xinxia.(2006).An l-glutamate amperometric biosensor based on redox polymer wired horseradish peroxidase and glutamate oxidase.Chinese journal of analytical chemistry,34,S1-s4.
MLA	Li Huaqing,et al."An l-glutamate amperometric biosensor based on redox polymer wired horseradish peroxidase and glutamate oxidase".Chinese journal of analytical chemistry 34(2006):S1-s4.

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