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	Effects of codon modification on human bmp2 gene expression in tobacco plants
	Suo, GL; Chen, B; Zhang, JY; Duan, ZY; He, ZQ; Yao, W; Yue, CY; Dai, JW
刊名	Plant cell reports
	2006-07-01
卷号	25期号:7页码:689-697
关键词	Codon optimization Codon usage Human bmp2 Transgenic tobacco
ISSN号	0721-7714
DOI	10.1007/s00299-006-0133-6
通讯作者	Dai, jw()
英文摘要	Bone morphogenetic protein 2 (bmp2) has great potential in therapeutic applications. we are working on generating transgenic plants as a bioreactor to produce bmp2. we have studied the effects of codon optimization on the expression of human bmp2 (hbmp2) in tobacco plants. three modified hbmp2 genes were transformed into tobacco under the control of either cauliflower mosaic virus 35s (camv35s) promoter or double-camv35s promoter plus alfalfa mosaic virus (amv) enhancer. the fused beta-glucuronidase (gus) reporter gene was used to facilitate the assay of protein expression. the results indicated that codon optimization could increase the protein expression level obviously under camv35s promoter. however, under relatively stronger initiation condition (double-camv35s promoter plus amv enhancer), only the gene with the lowest degree of codon optimization could increase the protein expression level. our findings suggest that the action of codon optimization may be influenced by the factors of promoter strength and a+t content in tobacco plants.
WOS关键词	BONE MORPHOGENETIC PROTEIN-2 ; MESSENGER-RNA STABILITY ; HIGH-LEVEL EXPRESSION ; BIAS ; VIRUS ; CELLS ; USAGE ; SITE ; DNA
WOS研究方向	Plant Sciences
WOS类目	Plant Sciences
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000238317700009
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2379583
专题	中国科学院大学
通讯作者	Dai, JW
作者单位	1.Chinese Acad Sci, Inst Genet & Dev Biol, Ctr Mol Dev Biol, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100080, Peoples R China 3.China Three Gorges Univ, Biotechnol Res Ctr, Yichang 443002, Peoples R China
推荐引用方式 GB/T 7714	Suo, GL,Chen, B,Zhang, JY,et al. Effects of codon modification on human bmp2 gene expression in tobacco plants[J]. Plant cell reports,2006,25(7):689-697.
APA	Suo, GL.,Chen, B.,Zhang, JY.,Duan, ZY.,He, ZQ.,...&Dai, JW.(2006).Effects of codon modification on human bmp2 gene expression in tobacco plants.Plant cell reports,25(7),689-697.
MLA	Suo, GL,et al."Effects of codon modification on human bmp2 gene expression in tobacco plants".Plant cell reports 25.7(2006):689-697.

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