Mechanism of Molecular Recognition on Tetrapetide-imprinted Monolith by Capillary Electrochromatography

	Mechanism of Molecular Recognition on Tetrapetide-imprinted Monolith by Capillary Electrochromatography
	Zheng Chao 1; Liu Zhao-Sheng 1; Gao Ru-Yu 2; Zhang Yu-Kui 2,3
刊名	chinese journal of chemistry
	2008-10-01
卷号	26 期号:10 页码:1857-1862
关键词	molecularly imprinted polymer monolith capillary electrochromatography YPLG (Tyr-Pro-Leu-Gly) recognition mechanism
通讯作者	gao ru-yu
英文摘要	a tetrapeptide-imprinted capillary monolithic column was synthesized successfully in the presence of yplg (tyr-pro-leu-gly) as template by in situ thermo-initiated polymerization. for exploring the recognition mechanism of molecularly imprinted polymer in a capillary electrochromatography (cec) mode, yplg and its structural analogue, ypgl (tyr-pro-gly-leu), differing in amino acid sequence, were used as model compounds. it is hard work to separate the two tetrapeptides by a simple cec or a capillary electrophoresis mode due to their similar chemical structures and values of pi. however, the results showed that yplg could be recognized specially by the imprinted monolith. the separation factor (alpha') and the resolution (r(s)) between yplg and ypgl were 1.73 and 3.72, respectively. the effects of organic modifier concentration, ph value of buffer, salt concentration of buffer and column temperature on the recognition of yplg and ypgl were investigated. a non-linear van't hoff behavior of the template was observed on a yplg-imprinted column, which suggested the presence of multiple types of retention mechanisms or multiple types of binding sites. the results showed that the molecular recognition on mip monolith in the cec mode mainly derived from hydrogen bonding between yplg and mip and three-dimensional structure of the imprinting cavities.
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, multidisciplinary
研究领域[WOS]	chemistry
关键词[WOS]	chiral recognition ; epitope approach ; polymers ; separation ; temperature ; column ; enantiomers ; gel ; retention ; oxytocin
收录类别	SCI
原文出处	false
语种	英语
WOS记录号	WOS:000260702500018
公开日期	2010-11-30
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/101413]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Tianjin Med Univ, Coll Pharm, Tianjin 300070, Peoples R China 2.Nankai Univ, State Key Lab Element Organ Chem, Inst Element Organ Chem, Tianjin 300071, Peoples R China 3.Chinese Acad Sci, Natl Chromatog Res & Anal Ctr, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Zheng Chao,Liu Zhao-Sheng,Gao Ru-Yu,et al. Mechanism of Molecular Recognition on Tetrapetide-imprinted Monolith by Capillary Electrochromatography[J]. chinese journal of chemistry,2008,26(10):1857-1862.
APA	Zheng Chao,Liu Zhao-Sheng,Gao Ru-Yu,&Zhang Yu-Kui.(2008).Mechanism of Molecular Recognition on Tetrapetide-imprinted Monolith by Capillary Electrochromatography.chinese journal of chemistry,26(10),1857-1862.
MLA	Zheng Chao,et al."Mechanism of Molecular Recognition on Tetrapetide-imprinted Monolith by Capillary Electrochromatography".chinese journal of chemistry 26.10(2008):1857-1862.