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	Adapting rice anther culture to gene transformation and rna interference
	Chen Caiyan; Xiao Han; Zhang Wenli; Wang Aiju; Xia Zhihui; Li Xiaobing; Zhai Wenxue; Heng Zhukuan; Zhu Lihuang
刊名	Science in china series c-life sciences
	2006-10-01
卷号	49期号:5页码:414-428
关键词	Rice (oryza sativa l.) Anther callus Agrobacterium-mediated transformation Rna interference Xa21 Osmads2
ISSN号	1006-9305
DOI	10.1007/s11427-006-2013-2
通讯作者	Zhu lihuang(lhzhu@genetics.ac.cn)
英文摘要	Anther culture offers a rapid method of generating homozygous lines for breeding program and genetic analysis. to produce homozygous transgenic lines of rice (oryza sativa l.) in one step, we developed an efficient protocol of anther-callus-based transformation mediated by agrobacterium after optimizing several factors influencing efficient transformation, including callus induction and agrobacterium density for co-cultivation. using this protocol, we obtained 145 independent green transformants from five cultivars of japonica rice by transformation with a binary vector pcxk1301 bearing the rice gene, xa21 for resistance to bacterial blight, of which 140 were further confirmed by pcr and southern hybridization analysis, including haploids (32.1%), diploids (62.1%) and mixoploids (7.5%). fifteen diploids were found to be doubled haploids, which accounted for 10.7% of the total positive lines. finally, by including 28 from colchicine induced or spontaneous diploiclization of haploids later after transformation, a total of 43 doubled haploids (30.7%) of xa21 transgenic lines were obtained. we also generated two rnai transgenic haploids of the rice osmads2 gene, a putative redundant gene of osmads4 based on their sequence similarity, to investigate its possible roles in rice flower development by this method. flowers from the two osmads2 rnai transgenic haploids displayed obvious homeotic alternations, in which lodicules were transformed into palea/lemma-like tissues, whereas identities of other floral organs were maintained. the phenotypic alternations were proved to result from specific transcriptional suppression of osmads2 gene by the introduced rnai transgene. the results confirmed that osmads2 is involved in lodicule development of rice flower and functionally redundant with osmads4 gene. our results demonstrated that rice anther culture could be adapted to gene transformation and rnai analysis in rice.
WOS关键词	AGROBACTERIUM-MEDIATED TRANSFORMATION ; FLORAL ORGAN IDENTITY ; DOUBLE-STRANDED-RNA ; T-DNA ; INDICA RICE ; TRANSGENIC PLANTS ; EFFICIENCY ; GENOME ; CHROMOSOMES ; INTEGRATION
WOS研究方向	Life Sciences & Biomedicine - Other Topics
WOS类目	Biology
语种	英语
出版者	SCIENCE CHINA PRESS
WOS记录号	WOS:000242641700002
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2379536
专题	中国科学院大学
通讯作者	Zhu Lihuang
作者单位	1.Chinese Acad Sci, State Key Lab Plant Genom, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Natl Ctr Plant Gene Res, Inst Genet & Dev Biol, Beijing 100101, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Chen Caiyan,Xiao Han,Zhang Wenli,et al. Adapting rice anther culture to gene transformation and rna interference[J]. Science in china series c-life sciences,2006,49(5):414-428.
APA	Chen Caiyan.,Xiao Han.,Zhang Wenli.,Wang Aiju.,Xia Zhihui.,...&Zhu Lihuang.(2006).Adapting rice anther culture to gene transformation and rna interference.Science in china series c-life sciences,49(5),414-428.
MLA	Chen Caiyan,et al."Adapting rice anther culture to gene transformation and rna interference".Science in china series c-life sciences 49.5(2006):414-428.

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