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	The expression, purification and crystallization of monomeric soluble human urokinase receptor
	Yuan, C; Huai, Q; Bian, CB; Huang, MD
刊名	Progress in biochemistry and biophysics
	2006-03-01
卷号	33期号:3页码:277-281
关键词	Soluble human urokinase receptor S2 cells Purification Crystallization
ISSN号	1000-3282
通讯作者	Huang, md()
英文摘要	Soluble form of human urokinase receptor was cloned into drosophila schneider 2 secretion expression vector pmt/bip/v5-his, and co-transfected with pcohygro into s2 cells, to establish stably s2 cells line. the expressed supar from such procedure tends to form oligomer and is unstable, presenting difficulties for its structural studies. here a purification procedure that yields monomeric supar was reported. supar obtained through such procedure is monomeric and quite stable. supar complex with amino terminal fragment (atf) of upa and an anti-supar antibody (atn615) was crystallized by dialysis method into diffracting crystals (1.9 angstrom).
WOS关键词	PLASMINOGEN-ACTIVATOR ; LOCALIZATION ; COMPLEX ; DOMAIN
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics
WOS类目	Biochemistry & Molecular Biology ; Biophysics
语种	英语
出版者	SCIENCE PRESS
WOS记录号	WOS:000236134000011
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2379528
专题	中国科学院大学
通讯作者	Huang, MD
作者单位	1.Chinese Acad Sci, Fujian Inst Res Struct Matter, State Key Lab Struct Chem, Fujian 350002, Peoples R China 2.Harvard Univ, Sch Med, Beth Israel Deaconess Med Ctr, Ctr Haemostasis & Thrombosis Res, Boston, MA 02215 USA 3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Yuan, C,Huai, Q,Bian, CB,et al. The expression, purification and crystallization of monomeric soluble human urokinase receptor[J]. Progress in biochemistry and biophysics,2006,33(3):277-281.
APA	Yuan, C,Huai, Q,Bian, CB,&Huang, MD.(2006).The expression, purification and crystallization of monomeric soluble human urokinase receptor.Progress in biochemistry and biophysics,33(3),277-281.
MLA	Yuan, C,et al."The expression, purification and crystallization of monomeric soluble human urokinase receptor".Progress in biochemistry and biophysics 33.3(2006):277-281.

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