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	Fusion of chemotactic peptide to a single-chain bi-specific antibody (scbsab) potentiates its cytotoxicity to target tumour cells
	Song, Jingzhen; Wang, Xiangbin; Lei, Chun; Piao, Jinhua; Yin, Changcheng; Zhang, Zhong; Lin, Qing; Huang, Hualiang
刊名	Biotechnology and applied biochemistry
	2006-11-01
卷号	45页码:147-154
关键词	Bi-specific antibody Chemotactic peptide Cytotoxicity Ovarian carcinoma Peripheral-blood lymphocyte Tumour-specific cytolysis
ISSN号	0885-4513
DOI	10.1042/ba20060060
通讯作者	Huang, hualiang(hlhuang@genetics.ac.cn)
英文摘要	Anti-tumour bsab (bi-specific antibody) has been proved very effective in killing tumour cells both in vitro and in vivo. in order to enhance its ability to recruit and activate t-lymphocytes and then improve tumour-specific cytolysis, an anti-ovarian carcinoma/cd3 bsab, bhl-i, was fused to n-terminal 18 peptide of ccl21 (cc chemokine ligand 21) to produce a new chemotactic bsab, named 18tbhl. it was expressed in soluble form in the cytoplasm of escherichia coli and purified with deae anion-exchange chromatography and immobilized-metal-ion affinity chromatography. the chemotactic ability of 18tbhl to pbls (peripheral-blood lymphocytes) was detected by boyden chamber chemotaxis assay. the specific ability to bind to ovarian carcinoma cells, skov3, and pbls was tested by elisa and flow cytometry. just as expected, the enhanced tumour-specific cytolysis of 18tbhl was validated by mtt [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2h-tetrazolium bromide] method and flow cytometry. the results indicated that fusion of chemotactic peptide to bsab potentiated its cytotoxicity to tumour cells in vitro. it suggests that 18tbhl may be a promising candidate agent in cancer immunotherapy.
WOS关键词	LYMPHOID-TISSUE CHEMOKINE ; IMMUNODEFICIENCY-VIRUS TYPE-1 ; N-TERMINAL PEPTIDES ; BISPECIFIC ANTIBODIES ; CC-CHEMOKINE ; FACTOR-I ; RECEPTOR-BINDING ; DENDRITIC CELLS ; T-LYMPHOCYTES ; PROTEIN
WOS研究方向	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS类目	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
语种	英语
出版者	PORTLAND PRESS LTD
WOS记录号	WOS:000241975100005
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2379421
专题	中国科学院大学
通讯作者	Huang, Hualiang
作者单位	1.Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China 2.Grad Univ, Chinese Acad Sci, Beijing 100049, Peoples R China 3.Beijing ABT Genet Engn Technol Co Ltd, Beijing 102206, Peoples R China
推荐引用方式 GB/T 7714	Song, Jingzhen,Wang, Xiangbin,Lei, Chun,et al. Fusion of chemotactic peptide to a single-chain bi-specific antibody (scbsab) potentiates its cytotoxicity to target tumour cells[J]. Biotechnology and applied biochemistry,2006,45:147-154.
APA	Song, Jingzhen.,Wang, Xiangbin.,Lei, Chun.,Piao, Jinhua.,Yin, Changcheng.,...&Huang, Hualiang.(2006).Fusion of chemotactic peptide to a single-chain bi-specific antibody (scbsab) potentiates its cytotoxicity to target tumour cells.Biotechnology and applied biochemistry,45,147-154.
MLA	Song, Jingzhen,et al."Fusion of chemotactic peptide to a single-chain bi-specific antibody (scbsab) potentiates its cytotoxicity to target tumour cells".Biotechnology and applied biochemistry 45(2006):147-154.

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