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	The effect of collagen-targeting platelet-derived growth factor on cellularization and vascularization of collagen scaffolds
	Lin, Hang; Chen, Bing; Sun, Wenjie; Zhao, Wenxue; Zhao, Yannan; Dai, Jianwu
刊名	Biomaterials
	2006-11-01
卷号	27期号:33页码:5708-5714
关键词	Platelet-derived growth factor bb (pdgf-bb) Collagen Collagen-targeting system Collagen-binding domain (cbd)
ISSN号	0142-9612
DOI	10.1016/j.biomaterials.2006.07.023
通讯作者	Dai, jianwu(jwdai@genetics.ac.cn)
英文摘要	Platelet-derived growth factor bb (pdgf-bb) was an important inductive factor during wound healing, but the lack of efficient delivery system limited its clinical application. here, a peptide of seven amino acids was firstly utilized to engineer pdgf-bb to construct a collagen-targeting system. this peptide functioned as collagen-binding domain (cbd) to specially target the pdgf-bb to the collagen and restrict its diffusion. in our study, in vitro activity assay showed that the cbd-fused pdgf (cbd-pdgf) and native pdgf (nat-pdgf) possessed similar activity to stimulate the human fibroblast proliferation. as expected, this peptide promoted the binding of pdgf to collagen scaffolds, and cbd-pdgf caused more cells to proliferate on the collagen gel than nat-pdgf when the same amounts of pdgf were added. in the in vivo experiment, cbd-pdgf-loaded collagen scaffolds were uniformly cellularized and vascularized compared to that of nat-pdgf-loaded scaffolds. our study demonstrated that the cbd targeted pdgf to the collagen scaffold to exert its functions, and it suggested this could be an effective system for targeting tissue regeneration and wound repair. (c) 2006 elsevier ltd. all rights reserved.
WOS关键词	FACTOR FUSION PROTEIN ; FACTOR DELIVERY ; BINDING DOMAIN ; RENATURATION ; OPPORTUNITIES ; EXPRESSION ; CHALLENGES ; TISSUES ; MATRIX ; MODEL
WOS研究方向	Engineering ; Materials Science
WOS类目	Engineering, Biomedical ; Materials Science, Biomaterials
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000241131500010
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2379389
专题	中国科学院大学
通讯作者	Dai, Jianwu
作者单位	1.Chinese Acad Sci, Key Lab Mol Dev Biol, Inst Genet & Dev Biol, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100080, Peoples R China
推荐引用方式 GB/T 7714	Lin, Hang,Chen, Bing,Sun, Wenjie,et al. The effect of collagen-targeting platelet-derived growth factor on cellularization and vascularization of collagen scaffolds[J]. Biomaterials,2006,27(33):5708-5714.
APA	Lin, Hang,Chen, Bing,Sun, Wenjie,Zhao, Wenxue,Zhao, Yannan,&Dai, Jianwu.(2006).The effect of collagen-targeting platelet-derived growth factor on cellularization and vascularization of collagen scaffolds.Biomaterials,27(33),5708-5714.
MLA	Lin, Hang,et al."The effect of collagen-targeting platelet-derived growth factor on cellularization and vascularization of collagen scaffolds".Biomaterials 27.33(2006):5708-5714.

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