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	Study on the antifertility effects of the plasmid dna vaccine expressing partial brldh-c4 '
	Chang, JJ; Peng, JP; Yang, Y; Wang, JL; Xu, L
刊名	Reproduction
	2006
卷号	131期号:1页码:183-192
ISSN号	1470-1626
DOI	10.1530/rep.1.00262
通讯作者	Peng, jp()
英文摘要	Partial cdna sequence coding for microtus brandti radde (brandt's vole) testes-specific lactate dehydrogenase (brldh-c4) was amplified by reverse transcription-polymerase chain reaction (rt-pcr). by inserting the product into the eukaryotic expression vector pcr3.1, pcr3.1-brldh-c4' was obtained as the prototype of contraceptive dna vaccine. immunization with pcr3.1-brldh-c4' in balb/c mice generated antibodies specific to purified brldh-c4' and native mouse ldh-c4 protein. the birth rate of the pcr3.1-brldh-c4' immunized mice was found to be decreased significantly (80% lower than that of those immunized with pcr3.1). functions of the elicited antibodies in sera from pcr3.1-brldh-c4' inoculated mice were further explored. the results indicated that the antibodies from the mice injected with pcr3.1-brldh-c4' could cause the agglutination of normal sperm suspension, while the ovarian structure and the development of ovarian follicles of these mice were not impaired, which gives a possible explanation for the immunocontraceptive effects of the pcr3.1-brldh-c4' dna vaccine.
WOS关键词	LACTATE-DEHYDROGENASE LDH-C4 ; T-CELL EPITOPE ; IMMUNE-RESPONSES ; SYNTHETIC EPITOPE ; FEMALE MICE ; IMMUNIZATION ; FERTILITY ; ISOZYME ; CONSTRUCTION ; INFERTILITY
WOS研究方向	Developmental Biology ; Reproductive Biology
WOS类目	Developmental Biology ; Reproductive Biology
语种	英语
出版者	BIO SCIENTIFICA LTD
WOS记录号	WOS:000235243200019
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2379260
专题	中国科学院大学
通讯作者	Peng, JP
作者单位	1.Chinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100080, Peoples R China
推荐引用方式 GB/T 7714	Chang, JJ,Peng, JP,Yang, Y,et al. Study on the antifertility effects of the plasmid dna vaccine expressing partial brldh-c4 '[J]. Reproduction,2006,131(1):183-192.
APA	Chang, JJ,Peng, JP,Yang, Y,Wang, JL,&Xu, L.(2006).Study on the antifertility effects of the plasmid dna vaccine expressing partial brldh-c4 '.Reproduction,131(1),183-192.
MLA	Chang, JJ,et al."Study on the antifertility effects of the plasmid dna vaccine expressing partial brldh-c4 '".Reproduction 131.1(2006):183-192.

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