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Screen-printed enzyme electrodes for detection of organophosphate pesticide residue
Zhang, J; Wang, YL; Yuan, ZB
刊名Acta chimica sinica
2006-03-14
卷号64期号:5页码:428-434
关键词Enzyme electrode Screen-printed Organophosphate pesticide residue Electrochemistry Acetylcholinesterase
ISSN号0567-7351
通讯作者Zhang, j(yuanzhb@gscas.ac.cn)
英文摘要A sensitive amperometric screen-printed enzyme electrode suitable for detection of organophosphate pesticide residue was developed, which can be operated at a low applied potential of 0.10 v. the first part of the study was focused on selected condition such as accession of the modified material and applied potential on electrode, optimization of the pretreatment time and response time, influence of environment temperature and ph of phosphate buffer solution, effect of substrate concentration, choice of immobilization method, effect of temperature and time when enzyme was immobilized on screen-printed electrodes. in the second part of the study, pesticide paraoxon-ethyl and reality sample were detected. the lowest detectable concentration in a standard solution was 2.10 ng circle ml(-1). the immobilization procedure can be easily adapted to the working electrode employed by electrochemical detectors. this novel method of screen-printed enzyme electrode is promising for the development of subminiature apparatus for measuring pesticide residues.
WOS关键词NERVE AGENTS ; BIOSENSOR ; ACETYLCHOLINESTERASE ; IMMOBILIZATION ; GLUCOSE ; ADSORPTION ; POLYMER ; OXIDASE ; CHOLINE ; ACID
WOS研究方向Chemistry
WOS类目Chemistry, Multidisciplinary
语种英语
出版者SCIENCE PRESS
WOS记录号WOS:000236189000013
内容类型期刊论文
URI标识http://www.corc.org.cn/handle/1471x/2379194
专题中国科学院大学
通讯作者Zhang, J
作者单位1.Chinese Acad Sci, Coll Chem & Chem Engn, Grad Sch, Beijing 100049, Peoples R China
2.Inner Mongolia Univ, Coll Chem & Chem Engn, Hohhot 010021, Peoples R China
推荐引用方式
GB/T 7714
Zhang, J,Wang, YL,Yuan, ZB. Screen-printed enzyme electrodes for detection of organophosphate pesticide residue[J]. Acta chimica sinica,2006,64(5):428-434.
APA Zhang, J,Wang, YL,&Yuan, ZB.(2006).Screen-printed enzyme electrodes for detection of organophosphate pesticide residue.Acta chimica sinica,64(5),428-434.
MLA Zhang, J,et al."Screen-printed enzyme electrodes for detection of organophosphate pesticide residue".Acta chimica sinica 64.5(2006):428-434.
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