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	A novel method to transfer gene in vivo system
	He Xin; Qi Bing; Liu Gui-Sheng; Yu Wei-Dong; Chen Qing-Xuan
刊名	Progress in biochemistry and biophysics
	2006-07-01
卷号	33期号:7页码:685-690
关键词	Testis injection Gene transfer In vivo Transgenic animal
ISSN号	1000-3282
通讯作者	Chen qing-xuan(qingxuanchen@yahoo.coma)
英文摘要	A new and effective method to produce transgenic animals was established. without a surgical incision, the recombinant plasmid containing green fluorescence protein (gfp) cdna was repeatedly injected into male mouse testis at multi-sites. after few weeks of the final injection, the injected male was mated with normal oestrus female to produce transgenic mice. the presence of the gfp cdna in f(1) transgenic individuals were detected by polymerase chain reaction and southern blot hybridization, which showed that the transgenic rate of mouse f(1) offspring was 41%. the transferred gene was integrated into the host genome and could be transmitted to its offspring. when the positive f(1) individuals were mated with the wild type icr mice, the, f(2) individuals had a transgenic rate of 37%. the results indicate that the high efficiency of gene transfer and the limited number of manipulations make the method suitable for creating a large number of transgenic animals, especially, for producing domestic animals.
WOS关键词	MOUSE SPERMATOGENIC CELLS ; FOREIGN DNA ; SPERM ; TESTIS ; MICE ; TRANSMISSION ; TRANSGENE ; EFFICIENT ; PROTEIN ; PIG
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics
WOS类目	Biochemistry & Molecular Biology ; Biophysics
语种	英语
出版者	CHINESE ACAD SCIENCES, INST BIOPHYSICS
WOS记录号	WOS:000239288700013
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2379119
专题	中国科学院大学
通讯作者	Chen Qing-Xuan
作者单位	1.Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	He Xin,Qi Bing,Liu Gui-Sheng,et al. A novel method to transfer gene in vivo system[J]. Progress in biochemistry and biophysics,2006,33(7):685-690.
APA	He Xin,Qi Bing,Liu Gui-Sheng,Yu Wei-Dong,&Chen Qing-Xuan.(2006).A novel method to transfer gene in vivo system.Progress in biochemistry and biophysics,33(7),685-690.
MLA	He Xin,et al."A novel method to transfer gene in vivo system".Progress in biochemistry and biophysics 33.7(2006):685-690.

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