Formation of calcium phosphates in gelatin with a novel diffusion system | |
Teng, SH; Shi, JJ; Chen, LJ | |
刊名 | Colloids and surfaces b-biointerfaces
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2006-04-15 | |
卷号 | 49期号:1页码:87-92 |
关键词 | Calcium phosphates Novel double diffusion system Gelatin Ph values Concentration |
ISSN号 | 0927-7765 |
DOI | 10.1016/j.colsurfb.2006.03.005 |
通讯作者 | Chen, lj() |
英文摘要 | The present paper demonstrated a novel and simple diffusion system to precipitate calcium phosphates in gelatin gel. in this system, a gelatin cup was specially used as the membrane separating reservoirs of calcium and phosphate ions. relative to the conventional diffusion system, the novel one in our experiment decreased the time required for the deposition from 5-7 days to 20 h and increased the amount of the precipitated mineral phases significantly. the influence of ph values and concentrations of calcium and phosphate solutions buffered with tris-hcl and naoh, respectively, was investigated. the results showed that precipitation of the mineral phase at low ph values (7 for calcium and 11 for phosphate) and concentrations (200 mm for calcium and 15 nm for phosphate) resulted in the formation of plate-like octacalcium phosphate (ocp) crystals. with increasing the ph values of calcium and phosphate solutions to 8 and 12, respectively, spherical amorphous calcium phosphate (acp) particles were obtained uniquely. furthermore, flower-like hydroxyapatite (hap) aggregates composed of many nano-sized needles were formed from the solutions with high ph values (8 for calcium and 12 for phosphate) and concentrations (500 mm for calcium and 37.5 mm for phosphate). the novel diffusion system is proposed to play an important role in both studying the process of biological mineralization and synthesizing calcium phosphates in different forms. (c) 2006 elsevier b.v. all rights reserved. |
WOS关键词 | HYDROXYAPATITE FORMATION ; COLLAGEN ; BIOMINERALIZATION ; PRECIPITATION ; PROTEOGLYCANS ; HYDROLYSIS ; TITANIUM ; CRYSTALS ; FIBRILS ; APATITE |
WOS研究方向 | Biophysics ; Chemistry ; Materials Science |
WOS类目 | Biophysics ; Chemistry, Physical ; Materials Science, Biomaterials |
语种 | 英语 |
出版者 | ELSEVIER SCIENCE BV |
WOS记录号 | WOS:000237652200013 |
内容类型 | 期刊论文 |
URI标识 | http://www.corc.org.cn/handle/1471x/2379113 |
专题 | 中国科学院大学 |
通讯作者 | Chen, LJ |
作者单位 | 1.Chinese Acad Sci, Tech Inst Phys & Chem, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
推荐引用方式 GB/T 7714 | Teng, SH,Shi, JJ,Chen, LJ. Formation of calcium phosphates in gelatin with a novel diffusion system[J]. Colloids and surfaces b-biointerfaces,2006,49(1):87-92. |
APA | Teng, SH,Shi, JJ,&Chen, LJ.(2006).Formation of calcium phosphates in gelatin with a novel diffusion system.Colloids and surfaces b-biointerfaces,49(1),87-92. |
MLA | Teng, SH,et al."Formation of calcium phosphates in gelatin with a novel diffusion system".Colloids and surfaces b-biointerfaces 49.1(2006):87-92. |
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