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	Formation of calcium phosphates in gelatin with a novel diffusion system
	Teng, SH; Shi, JJ; Chen, LJ
刊名	Colloids and surfaces b-biointerfaces
	2006-04-15
卷号	49期号:1页码:87-92
关键词	Calcium phosphates Novel double diffusion system Gelatin Ph values Concentration
ISSN号	0927-7765
DOI	10.1016/j.colsurfb.2006.03.005
通讯作者	Chen, lj()
英文摘要	The present paper demonstrated a novel and simple diffusion system to precipitate calcium phosphates in gelatin gel. in this system, a gelatin cup was specially used as the membrane separating reservoirs of calcium and phosphate ions. relative to the conventional diffusion system, the novel one in our experiment decreased the time required for the deposition from 5-7 days to 20 h and increased the amount of the precipitated mineral phases significantly. the influence of ph values and concentrations of calcium and phosphate solutions buffered with tris-hcl and naoh, respectively, was investigated. the results showed that precipitation of the mineral phase at low ph values (7 for calcium and 11 for phosphate) and concentrations (200 mm for calcium and 15 nm for phosphate) resulted in the formation of plate-like octacalcium phosphate (ocp) crystals. with increasing the ph values of calcium and phosphate solutions to 8 and 12, respectively, spherical amorphous calcium phosphate (acp) particles were obtained uniquely. furthermore, flower-like hydroxyapatite (hap) aggregates composed of many nano-sized needles were formed from the solutions with high ph values (8 for calcium and 12 for phosphate) and concentrations (500 mm for calcium and 37.5 mm for phosphate). the novel diffusion system is proposed to play an important role in both studying the process of biological mineralization and synthesizing calcium phosphates in different forms. (c) 2006 elsevier b.v. all rights reserved.
WOS关键词	HYDROXYAPATITE FORMATION ; COLLAGEN ; BIOMINERALIZATION ; PRECIPITATION ; PROTEOGLYCANS ; HYDROLYSIS ; TITANIUM ; CRYSTALS ; FIBRILS ; APATITE
WOS研究方向	Biophysics ; Chemistry ; Materials Science
WOS类目	Biophysics ; Chemistry, Physical ; Materials Science, Biomaterials
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000237652200013
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2379113
专题	中国科学院大学
通讯作者	Chen, LJ
作者单位	1.Chinese Acad Sci, Tech Inst Phys & Chem, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Teng, SH,Shi, JJ,Chen, LJ. Formation of calcium phosphates in gelatin with a novel diffusion system[J]. Colloids and surfaces b-biointerfaces,2006,49(1):87-92.
APA	Teng, SH,Shi, JJ,&Chen, LJ.(2006).Formation of calcium phosphates in gelatin with a novel diffusion system.Colloids and surfaces b-biointerfaces,49(1),87-92.
MLA	Teng, SH,et al."Formation of calcium phosphates in gelatin with a novel diffusion system".Colloids and surfaces b-biointerfaces 49.1(2006):87-92.

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