QQ客服 官方微博 反馈留言

	Berberine inhibits 3t3-l1 adipocyte differentiation through the ppar gamma pathway
	Huang, Cheng; Zhang, Yuebo; Gong, Zhenwei; Sheng, Xiaoyan; Li, Zongmeng; Zhang, Wei; Qin, Ying
刊名	Biochemical and biophysical research communications
	2006-09-22
卷号	348期号:2页码:571-578
关键词	Berberine 3t3-l1 Ppar gamma Adipocyte differentiation Obesity Diabetes
ISSN号	0006-291X
DOI	10.1016/j.bbrc.2006.07.095
通讯作者	Qin, ying(yqin@sibs.ac.cn)
英文摘要	Berberine (bbr), a compound purified from cortidis rhizoma, reduces serum cholesterol, triglycerides, and ldl-cholesterol of hyper-cholesterolemic patients and high fat diet fed animals, and increases hepatic ldlr mrna and protein levels through a post-transcriptional mechanism. bbr also enhances the hypoglycemic action of insulin in diabetic animal models. here, we show that bbr inhibits the differentiation of 3t3-l1 preadipocytes induced by dm and suppresses the mitotic clonal expansion of 3t3-l1 preadipocytes in a time-and dose-dependent manner. gene expression analysis and western blot analysis reveal that the bbr inhibits the mrna and protein levels of adipogenesis related transcription factors ppar gamma and c/ebp alpha and their upstream regulator, c/ebp beta. reporter gene assays demonstrate that the full-length ppar gamma and alpha transcription activities are inhibited by bbr. using real-time pcr, we have also found that the ppar target genes that are involved in adipocyte differentiation, such as ap2, cd36, aco, lpl, and other adipocyte markers, are suppressed by bbr. these studies suggest that bbr works on multiple molecular targets as an inhibitor of ppar gamma and alpha, and is a potential weight reducing, hypolipidemic, and hypoglycemic drug. (c) 2006 elsevier inc. all rights reserved.
WOS关键词	MITOTIC CLONAL EXPANSION ; ACTIVATED RECEPTOR-GAMMA ; C/EBP-BETA ; ADIPOGENESIS ; EXPRESSION ; MECHANISM ; CELLS ; TRANSCRIPTION ; FIBROBLASTS ; AGONISTS
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics
WOS类目	Biochemistry & Molecular Biology ; Biophysics
语种	英语
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
WOS记录号	WOS:000240275000033
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2379090
专题	中国科学院大学
通讯作者	Qin, Ying
作者单位	Chinese Acad Sci, Grad Sch, Shanghai Inst Biol Sci, Inst Nutr Sci, Shanghai 200031, Peoples R China
推荐引用方式 GB/T 7714	Huang, Cheng,Zhang, Yuebo,Gong, Zhenwei,et al. Berberine inhibits 3t3-l1 adipocyte differentiation through the ppar gamma pathway[J]. Biochemical and biophysical research communications,2006,348(2):571-578.
APA	Huang, Cheng.,Zhang, Yuebo.,Gong, Zhenwei.,Sheng, Xiaoyan.,Li, Zongmeng.,...&Qin, Ying.(2006).Berberine inhibits 3t3-l1 adipocyte differentiation through the ppar gamma pathway.Biochemical and biophysical research communications,348(2),571-578.
MLA	Huang, Cheng,et al."Berberine inhibits 3t3-l1 adipocyte differentiation through the ppar gamma pathway".Biochemical and biophysical research communications 348.2(2006):571-578.

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。