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	Inhibitory activity of chlorogenic acid on enzymes involved in the fatty acid synthesis in animals and bacteria
	Li, BH; Ma, XF; Wu, XD; Tian, WX
刊名	Iubmb life
	2006
卷号	58期号:1页码:39-46
关键词	Fatty acid synthase Fabg Chlorogenic acid Inhibition Kinetic
ISSN号	1521-6543
DOI	10.1080/15216540500507408
通讯作者	Tian, wx(tianweixi@gucas.ac.cn)
英文摘要	It was found that chlorogenic acid inhibited in vitro animal fatty acid synthase (fas i) and the beta-ketoacyl-acp reductase (fabg) from escherichia coli in a concentration-dependent manner with respective ic50 of 94.8 and 88.1 mu m. the results of lineweaver-burk plots indicated that chlorogenic acid inhibited competitively the binding of nadph to fas i, while left those of acetyl-coa and malonyl-coa unaffected. further kinetic studies showed that chlorogenic acid blocked the activity of fas i mainly by inhibiting the beta-ketoacyl reductase domain, which catalyzed the same reaction as that done by fabg in the fatty acid synthesis. the beta-ketoacyl reduction reactions accomplished by both fas i and fabg required nucleotide cofactor, nadph. furthermore, the lineweaver-burk and yonetani-theorell analyses implicated that chlorogenic acid filled competitively in the binding-pocket of nadph in the beta-ketoacyl reductase domain of fas i. the similar results were also obtained from the inhibition of fabg by chlorogenic acid. as observed in these results, the inhibitions of fas i and fabg by chlorogenic acid were highly related to the interference of the inhibitor with nadph, which was possibly due to the similarity between chlorogenic acid and some portion of nadph, maybe the section consisting of the two ribose groups.
WOS关键词	ACYL CARRIER PROTEIN ; ESCHERICHIA-COLI ; EPIGALLOCATECHIN GALLATE ; SYNTHASE INHIBITORS ; NATURAL INHIBITOR ; CHICKEN LIVER ; CANCER CELLS ; FOOD-INTAKE ; BIOSYNTHESIS ; TEA
WOS研究方向	Biochemistry & Molecular Biology ; Cell Biology
WOS类目	Biochemistry & Molecular Biology ; Cell Biology
语种	英语
出版者	WILEY-BLACKWELL
WOS记录号	WOS:000235981500006
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2379072
专题	中国科学院大学
通讯作者	Tian, WX
作者单位	Grad Univ Chinese Acad Sci, Dept Biol, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Li, BH,Ma, XF,Wu, XD,et al. Inhibitory activity of chlorogenic acid on enzymes involved in the fatty acid synthesis in animals and bacteria[J]. Iubmb life,2006,58(1):39-46.
APA	Li, BH,Ma, XF,Wu, XD,&Tian, WX.(2006).Inhibitory activity of chlorogenic acid on enzymes involved in the fatty acid synthesis in animals and bacteria.Iubmb life,58(1),39-46.
MLA	Li, BH,et al."Inhibitory activity of chlorogenic acid on enzymes involved in the fatty acid synthesis in animals and bacteria".Iubmb life 58.1(2006):39-46.

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