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	Identification of atent3 as the main transporter for uridine uptake in arabidopsis roots
	Chen, KL; Xu, MX; Li, GY; Liang, H; Xia, ZL; Liu, X; Zhang, JS; Zhang, AM; Wang, DW
刊名	Cell research
	2006-04-01
卷号	16期号:4页码:377-388
关键词	Arabidopsis Equilibrative nucleoside transporter Expression pattern Nucleoside transport Functional analysis
ISSN号	1001-0602
DOI	10.1038/sj.cr.7310049
通讯作者	Wang, dw()
英文摘要	Previous studies have shown that arabidopsis equilibrative nucleoside transporters (atents) possess transport activities when produced in yeast cells and are differentially expressed in arabidopsis organs. herein, we report further analysis on the nucleoside transport activities and transcriptional patterns of atent members. the recombinant proteins of atents 3, 6, and 7, but not those of atents 1, 2, 4, and 8, were found to transport thymidine with high affinity. contrary to previous suggestion that atent 1 may not transport uridine, this work showed that recombinant atent 1 was a ph-dependent and high-affinity transporter of uridine. when grown on ms plates, the atent3 knockout plants were more tolerant to the cytotoxic uridine analog 5-fluorouridine than wild-type plants and the knockout plants of atent 1 or atent6. consistent with this observation, the atent3 knockout line exhibited a significantly decreased ability to take up [h-3]uridine via the roots when compared with wild-type plants and the plants with mutated atent 1 or atent6. this indicates that atent3, but not atents 1 and 6, is the main transporter for uridine uptake in arabidopsis roots. the transcription of atents 1, 3, 4, 6, 7, and 8, was regulated in a complex manner during leaf development and senescence. in contrast, the six atent members were coordinately induced during seed germination. this work provides new information on the transport properties of recombinant atent proteins and new clues for future studies of the in vivo transport activities and physiological functions of the different ent proteins in arabidopsis plants.
WOS关键词	EQUILIBRATIVE NUCLEOSIDE TRANSPORTER ; SPRUCE PICEA-GLAUCA ; SACCHAROMYCES-CEREVISIAE ; INTRACELLULAR MEMBRANES ; FUNCTIONAL-CHARACTERIZATION ; PYRIMIDINE NUCLEOTIDE ; CYTOKININ TRANSPORT ; MOLECULAR-BIOLOGY ; THYMIDINE KINASE ; MAMMALIAN-CELLS
WOS研究方向	Cell Biology
WOS类目	Cell Biology
语种	英语
出版者	INST BIOCHEMISTRY & CELL BIOLOGY
WOS记录号	WOS:000237837400006
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2379033
专题	中国科学院大学
通讯作者	Wang, DW
作者单位	1.Chinese Acad Sci, Ctr Plant Gene Res Beijing, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Plant Cell & Chromosome Engn, Beijing 100101, Peoples R China 3.NW Agr & Forestry Univ, Sch Life Sci, Yangling 712100, Peoples R China 4.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Chen, KL,Xu, MX,Li, GY,et al. Identification of atent3 as the main transporter for uridine uptake in arabidopsis roots[J]. Cell research,2006,16(4):377-388.
APA	Chen, KL.,Xu, MX.,Li, GY.,Liang, H.,Xia, ZL.,...&Wang, DW.(2006).Identification of atent3 as the main transporter for uridine uptake in arabidopsis roots.Cell research,16(4),377-388.
MLA	Chen, KL,et al."Identification of atent3 as the main transporter for uridine uptake in arabidopsis roots".Cell research 16.4(2006):377-388.

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