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	Inactivation mechanism of the beta-ketoacyl-[acyl carrier protein] reductase of bacterial type-ii fatty acid synthase by epigallocatechin gallate
	Li, Bing-Hui; Zhang, Rui; Du, Ya-Tao; Sun, Ying-Hui; Tian, Wei-Xi
刊名	Biochemistry and cell biology-biochimie et biologie cellulaire
	2006-10-01
卷号	84期号:5页码:755-762
关键词	Fabg Egcg Segregation Inactivation
ISSN号	0829-8211
DOI	10.1139/o06-047
通讯作者	Tian, wei-xi(tianweixi@gucas.ac.cn)
英文摘要	Epipilocatechin gallate (egcg), a major compound from green tea, reversibly inhibits beta-ketoacyl-[acyl carrier protein] reductase (fabg) from escherichia coli. in this study, we found that egcg exhibited an atypical time-dependent inhibition of fabg, which possibly resulted from the egcg-induced aggregation of fabg. it was observed that fabg inactivation and aggregation occurred nearly simultaneously, with a lag time that decreased with increasing egcg concentration. these results suggest that some chemical reactions, required for aggregation and inactivation, occurred during the lag time. since egc was detected by hplc after the incubation of egcg with fabg, egcg probably covalently modified fabg. these further results showed that i tetramer of fabg must be modified by several, possibly 4, egcg molecules before the formation of fabg aggregates. fabg aggregation was a first-order reaction independent of protein concentration. due to an initial lag time, the first-order rate of aggregation gradually increased, reaching a maximal and constant value. the effect of increasing concentration of egcg on the first-order rate constant for aggregation indicated that egcg bound to fabg by affinity labeling. based on the results, we propose a mechanism for the interaction of egcg with fab-g:egcg first binds reversibly to each subunit of fabg, followed by covalent modification and then aggregation of the 4 egcg-modified subunits.
WOS关键词	ESCHERICHIA-COLI ; PROTEIN REDUCTASE ; BRASSICA-NAPUS ; BIOSYNTHESIS ; POLYPHENOLS ; TYPHIMURIUM ; ELONGATION ; FABG
WOS研究方向	Biochemistry & Molecular Biology ; Cell Biology
WOS类目	Biochemistry & Molecular Biology ; Cell Biology
语种	英语
出版者	NATL RESEARCH COUNCIL CANADA-N R C RESEARCH PRESS
WOS记录号	WOS:000242894900011
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2378995
专题	中国科学院大学
通讯作者	Tian, Wei-Xi
作者单位	Grad Univ, Chinese Acad Sci, Dept Biol, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Li, Bing-Hui,Zhang, Rui,Du, Ya-Tao,et al. Inactivation mechanism of the beta-ketoacyl-[acyl carrier protein] reductase of bacterial type-ii fatty acid synthase by epigallocatechin gallate[J]. Biochemistry and cell biology-biochimie et biologie cellulaire,2006,84(5):755-762.
APA	Li, Bing-Hui,Zhang, Rui,Du, Ya-Tao,Sun, Ying-Hui,&Tian, Wei-Xi.(2006).Inactivation mechanism of the beta-ketoacyl-[acyl carrier protein] reductase of bacterial type-ii fatty acid synthase by epigallocatechin gallate.Biochemistry and cell biology-biochimie et biologie cellulaire,84(5),755-762.
MLA	Li, Bing-Hui,et al."Inactivation mechanism of the beta-ketoacyl-[acyl carrier protein] reductase of bacterial type-ii fatty acid synthase by epigallocatechin gallate".Biochemistry and cell biology-biochimie et biologie cellulaire 84.5(2006):755-762.

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