Amperometric enzyme biosensor for the determination of high concentration lactate in whole blood of athletes | |
Liu, Chunxiu; Guo, Zonghui; Wang, Hui; Xu, Jianfang; Feng, Lianshi; Cai, Xinxia | |
刊名 | Rare metal materials and engineering
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2006-12-01 | |
卷号 | 35页码:83-86 |
关键词 | Biosensors Lactate oxidase High concentration lactate Whole blood |
ISSN号 | 1002-185X |
通讯作者 | Cai, xinxia(xxcai@mail.ie.ac.cn) |
英文摘要 | Amperometric biosensor applied to the determination of high concentration lactate in serum and whole blood was described. the biosensor was constructed by gold electrode modified with nanoplatinum particles. lactate oxidase (e.c. 1. 1.3.2) was immobilized at platinized activated gold electrode which was used for the determination of high concentration lactate at low potential (+0.2 v). the linear calibration graphs were obtained from 1 to 21 mmol(.)l(-1) lactate in serum and from 0.9 to 13.2 mmol(.)l(-1) lactate in whole blood. the correlation coefficients were 0.99 and 0.97, respectively at a steady-state response time of 50 s. |
WOS关键词 | FE(CN)(6)(3-) |
WOS研究方向 | Materials Science ; Metallurgy & Metallurgical Engineering |
WOS类目 | Materials Science, Multidisciplinary ; Metallurgy & Metallurgical Engineering |
语种 | 英语 |
出版者 | NORTHWEST INST NONFERROUS METAL RESEARCH |
WOS记录号 | WOS:000244457000026 |
内容类型 | 期刊论文 |
URI标识 | http://www.corc.org.cn/handle/1471x/2378967 |
专题 | 中国科学院大学 |
通讯作者 | Cai, Xinxia |
作者单位 | 1.Chinese Acad Sci, Inst Elect, State Key Lab Transducer Technol, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100080, Peoples R China 3.China Inst Sport Sci, Ctr Biol, Beijing 100061, Peoples R China |
推荐引用方式 GB/T 7714 | Liu, Chunxiu,Guo, Zonghui,Wang, Hui,et al. Amperometric enzyme biosensor for the determination of high concentration lactate in whole blood of athletes[J]. Rare metal materials and engineering,2006,35:83-86. |
APA | Liu, Chunxiu,Guo, Zonghui,Wang, Hui,Xu, Jianfang,Feng, Lianshi,&Cai, Xinxia.(2006).Amperometric enzyme biosensor for the determination of high concentration lactate in whole blood of athletes.Rare metal materials and engineering,35,83-86. |
MLA | Liu, Chunxiu,et al."Amperometric enzyme biosensor for the determination of high concentration lactate in whole blood of athletes".Rare metal materials and engineering 35(2006):83-86. |
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