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Amperometric enzyme biosensor for the determination of high concentration lactate in whole blood of athletes
Liu, Chunxiu; Guo, Zonghui; Wang, Hui; Xu, Jianfang; Feng, Lianshi; Cai, Xinxia
刊名Rare metal materials and engineering
2006-12-01
卷号35页码:83-86
关键词Biosensors Lactate oxidase High concentration lactate Whole blood
ISSN号1002-185X
通讯作者Cai, xinxia(xxcai@mail.ie.ac.cn)
英文摘要Amperometric biosensor applied to the determination of high concentration lactate in serum and whole blood was described. the biosensor was constructed by gold electrode modified with nanoplatinum particles. lactate oxidase (e.c. 1. 1.3.2) was immobilized at platinized activated gold electrode which was used for the determination of high concentration lactate at low potential (+0.2 v). the linear calibration graphs were obtained from 1 to 21 mmol(.)l(-1) lactate in serum and from 0.9 to 13.2 mmol(.)l(-1) lactate in whole blood. the correlation coefficients were 0.99 and 0.97, respectively at a steady-state response time of 50 s.
WOS关键词FE(CN)(6)(3-)
WOS研究方向Materials Science ; Metallurgy & Metallurgical Engineering
WOS类目Materials Science, Multidisciplinary ; Metallurgy & Metallurgical Engineering
语种英语
出版者NORTHWEST INST NONFERROUS METAL RESEARCH
WOS记录号WOS:000244457000026
内容类型期刊论文
URI标识http://www.corc.org.cn/handle/1471x/2378967
专题中国科学院大学
通讯作者Cai, Xinxia
作者单位1.Chinese Acad Sci, Inst Elect, State Key Lab Transducer Technol, Beijing 100080, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100080, Peoples R China
3.China Inst Sport Sci, Ctr Biol, Beijing 100061, Peoples R China
推荐引用方式
GB/T 7714
Liu, Chunxiu,Guo, Zonghui,Wang, Hui,et al. Amperometric enzyme biosensor for the determination of high concentration lactate in whole blood of athletes[J]. Rare metal materials and engineering,2006,35:83-86.
APA Liu, Chunxiu,Guo, Zonghui,Wang, Hui,Xu, Jianfang,Feng, Lianshi,&Cai, Xinxia.(2006).Amperometric enzyme biosensor for the determination of high concentration lactate in whole blood of athletes.Rare metal materials and engineering,35,83-86.
MLA Liu, Chunxiu,et al."Amperometric enzyme biosensor for the determination of high concentration lactate in whole blood of athletes".Rare metal materials and engineering 35(2006):83-86.
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