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	Molecular cloning and characterization of phytoene synthase gene from a unicellular green alga haematococcus pluvialis
	Liang Cheng-Wei; Zhao Fang-Qing; Qin Song; Tan Cong-Ping; Wei Wei; Meng Chun-Xiao
刊名	Progress in biochemistry and biophysics
	2006-09-01
卷号	33期号:9页码:854-860
关键词	Haematococcus pluvialis Phytoene synthase 5 ' flanking region Phylogenetic analysis Promoter
ISSN号	1000-3282
通讯作者	Qin song(sqin@ms.qdio.ac.cn)
英文摘要	The unicellular green alga haematococcus pluvialis accumulates a highly valuable ketocarotenoid, i.e. astaxanthin up to 4% dry weight under stress conditions. phytoene synthase is considered to be the first rate limiting enzyme in carotenoid biosynthesis pathway in h. pluvialis. the cdna and genomic genes of phytoene synthase, i.e. psy from h.pluvialis were cloned and characterized. result showed that psy had one open reading frame of 1 200 bp encoding a putative polypeptide of 400 amino acids which was interrupted by four introns. phylogenetic analysis revealed that psy from green algae formed a monophyletic clade, and its closer relationship was higher plants. by using genomic walking approach, an approximate 1 kb 5' flanking region of psy gene was cloned and a number of putative cis-regulatory elements were revealed. fusing a 297 bp internal sequence (-297 to -1 bp from the translation initiation codon of psy) with the reporter gene, i.e. lacz before attempted introducing the construct into the green alga via particle bombardment resulted in lacz transient expression.
WOS关键词	CAROTENOID BIOSYNTHESIS GENES ; MULTIPLE SEQUENCE ALIGNMENT ; CIS-ACTING ELEMENTS ; BETA-CAROTENE ; FUNCTIONAL-ANALYSIS ; DEHYDRATION STRESS ; DUNALIELLA-SALINA ; ARABIDOPSIS GENE ; IN-VIVO ; ASTAXANTHIN
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics
WOS类目	Biochemistry & Molecular Biology ; Biophysics
语种	英语
出版者	CHINESE ACAD SCIENCES, INST BIOPHYSICS
WOS记录号	WOS:000240833200008
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2378913
专题	中国科学院大学
通讯作者	Qin Song
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Grad Univ Chinese Acad Sci, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Liang Cheng-Wei,Zhao Fang-Qing,Qin Song,et al. Molecular cloning and characterization of phytoene synthase gene from a unicellular green alga haematococcus pluvialis[J]. Progress in biochemistry and biophysics,2006,33(9):854-860.
APA	Liang Cheng-Wei,Zhao Fang-Qing,Qin Song,Tan Cong-Ping,Wei Wei,&Meng Chun-Xiao.(2006).Molecular cloning and characterization of phytoene synthase gene from a unicellular green alga haematococcus pluvialis.Progress in biochemistry and biophysics,33(9),854-860.
MLA	Liang Cheng-Wei,et al."Molecular cloning and characterization of phytoene synthase gene from a unicellular green alga haematococcus pluvialis".Progress in biochemistry and biophysics 33.9(2006):854-860.

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