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	Mitochondrial reactive oxygen species and nitric oxide-mediated cancer cell apoptosis in 2-butylamino-2-demethoxyhypocrellin b photodynamic treatment
	Lu, Zhongbing; Tao, Yi; Zhou, Zhixiang; Zhang, Junjing; Li, Cong; Ou, Lingcheng; Zhao, Baolu
刊名	Free radical biology and medicine
	2006-11-15
卷号	41期号:10页码:1590-1605
关键词	Reactive oxygen species Nitric oxide Mitochondrial nitric oxide synthase Photodynamic therapy Cytochrome c
ISSN号	0891-5849
DOI	10.1016/j.freeradbiomed.2006.08.021
通讯作者	Zhao, baolu(zhaobl@sun5.ibp.ac.cn)
英文摘要	Photodynamic therapy (pdt) is a novel and promising cancer treatment which employs a combination of a photosensitizing chemical and visible light to induce apoptosis in cancer cells. singlet oxygen has been recognized as the main origin of oxidative stress in pdt. however, the precise mechanism of pdt-induced apoptosis is not well characterized, especially the dualistic role of nitric oxide (no). to dissect the apoptosis pathways triggered by pdt, the intracellular free radicals in mcf-7 cells were investigated by examining a novel photosensitizer 2-butylamino-2-demethoxyhypocrellin b (2-ba-2-dmhb)-mediated pdt. it was found that exposure of the cells to 2-ba-2-dmhb and irradiation resulted in a significant increase of intracellular ros in minutes, and then followed by cytoplasmic free calcium enhancement, mitochondrial nitric oxide synthase (mtnos) activation, cytochrome c release, and apoptotic death. scavengers of singlet oxygen or no could attenuate pdt-induced cell viability loss, nucleus morphology changes, cytochrome c release, mitochondria swelling, and apo-apoptosis gene p53 and p21 mrna levels. the results suggested that both ros and no played important roles in the apoptosis-induced by pdt. (c) 2006 elsevier inc. all rights reserved.
WOS关键词	CYTOPLASMIC FREE CALCIUM ; CYTOCHROME-C RELEASE ; HYDROGEN-PEROXIDE ; 5-AMINOLEVULINIC ACID ; SYNTHASE ACTIVITY ; FREE-RADICALS ; TUMOR-CELLS ; HELA-CELLS ; RAT-BRAIN ; THERAPY
WOS研究方向	Biochemistry & Molecular Biology ; Endocrinology & Metabolism
WOS类目	Biochemistry & Molecular Biology ; Endocrinology & Metabolism
语种	英语
出版者	ELSEVIER SCIENCE INC
WOS记录号	WOS:000241563000009
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2378835
专题	中国科学院大学
通讯作者	Zhao, Baolu
作者单位	1.Chinese Acad Sci, Inst Biophys, State Key Lab Brain & Recognit Lab, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100029, Peoples R China 3.Univ Yuannan, Dept Chem, Kunming, Yunnan, Peoples R China 4.Shanghai Univ Trad Chinese Med, E Inst Shanghai Univ, Div Nitr Oxide & Inflammatory Med, Shanghai, Peoples R China
推荐引用方式 GB/T 7714	Lu, Zhongbing,Tao, Yi,Zhou, Zhixiang,et al. Mitochondrial reactive oxygen species and nitric oxide-mediated cancer cell apoptosis in 2-butylamino-2-demethoxyhypocrellin b photodynamic treatment[J]. Free radical biology and medicine,2006,41(10):1590-1605.
APA	Lu, Zhongbing.,Tao, Yi.,Zhou, Zhixiang.,Zhang, Junjing.,Li, Cong.,...&Zhao, Baolu.(2006).Mitochondrial reactive oxygen species and nitric oxide-mediated cancer cell apoptosis in 2-butylamino-2-demethoxyhypocrellin b photodynamic treatment.Free radical biology and medicine,41(10),1590-1605.
MLA	Lu, Zhongbing,et al."Mitochondrial reactive oxygen species and nitric oxide-mediated cancer cell apoptosis in 2-butylamino-2-demethoxyhypocrellin b photodynamic treatment".Free radical biology and medicine 41.10(2006):1590-1605.

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