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	An amperometric bienzyme biosensor for rapid measurement of alanine aminotransferase in whole blood
	Li, Huaqing; Guo, Zonghui; Wang, Hui; Cui, Dafu; Cai, Xinxia
刊名	Sensors and actuators b-chemical
	2006-12-07
卷号	119期号:2页码:419-424
关键词	Redox hydrogel Amperometric Biosensor Alanine aminotransferase L-glutamate oxidase Horseradish peroxidase
ISSN号	0925-4005
DOI	10.1016/j.snb.2005.12.041
通讯作者	Cai, xinxia(xxcai@mail.ie.ac.cn)
英文摘要	A two-electrode configuration has been fabricated by depositing a gold film on a polyethylene terephthalate (pet) substrate. the integrated reference electrode was obtained by screen-printing commercial silver and silver chloride paste on the gold electrode. the working electrode was modified by drop-coating a redox polymer of poly(4-vinylpyrodine) complexed with osmium (2,2'-bipyrridine)(2). the bienzymes (horseradish peroxidase and l-glutamate oxidase) were immobilized in the redox hydrogel matrices by the poly (ethylene glycol) diglycidyl ether (pegdge) crosslinker. the optimized biosensor was used for the amperometric measurement of l-glutamate in a stirred solution at 0 mv versus ag vertical bar agcl. a linear calibration graph was obtained in the concentration range of l-glutamate from 1 to 500 mu m (linear regression coefficient = 0.99) with a steady-state response time of less than 10 s. the sensors were characterized by a sensitivity of 55.3 mu a mm(-1) cm(-2) with an operational stability of more than 90% of its original activity over a period of 1 month. the alanine aminotransferase in real human serum and whole blood samples was analyzed subsequently with this sensor method and compared to the clinical method with satisfactory results. (c) 2006 elsevier b.v. all rights reserved.
WOS关键词	GLUTAMATE-PYRUVATE TRANSAMINASE ; COMPLEX-BILAYER MEMBRANE ; REFERENCE ELECTRODES ; OXIDASE ; SENSOR ; SERUM
WOS研究方向	Chemistry ; Electrochemistry ; Instruments & Instrumentation
WOS类目	Chemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation
语种	英语
出版者	ELSEVIER SCIENCE SA
WOS记录号	WOS:000241197600011
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2378793
专题	中国科学院大学
通讯作者	Cai, Xinxia
作者单位	1.Chinese Acad Sci, Inst Elect, State Key Lab Transducer Technol, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100080, Peoples R China
推荐引用方式 GB/T 7714	Li, Huaqing,Guo, Zonghui,Wang, Hui,et al. An amperometric bienzyme biosensor for rapid measurement of alanine aminotransferase in whole blood[J]. Sensors and actuators b-chemical,2006,119(2):419-424.
APA	Li, Huaqing,Guo, Zonghui,Wang, Hui,Cui, Dafu,&Cai, Xinxia.(2006).An amperometric bienzyme biosensor for rapid measurement of alanine aminotransferase in whole blood.Sensors and actuators b-chemical,119(2),419-424.
MLA	Li, Huaqing,et al."An amperometric bienzyme biosensor for rapid measurement of alanine aminotransferase in whole blood".Sensors and actuators b-chemical 119.2(2006):419-424.

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