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	A catalog for transcripts in the venom gland of the agkistrodon acutus: identification of the toxins potentially involved in coagulopathy
	Liu, QH; Zhang, XW; Wei, Y; Li, CJ; Huang, YJ; Qiu, PX; Su, XW; Hu, SN; Yan, GM
刊名	Biochemical and biophysical research communications
	2006-03-10
卷号	341期号:2页码:522-531
关键词	Agkistrodon acutus Coagulopathy Expressed sequence tags Toxins Transcriptome Venom gland
ISSN号	0006-291X
DOI	10.1016/j.bbrc.2006.01.006
通讯作者	Yan, gm()
英文摘要	Agkistrodon acutus is a special agkistrodon halys, only distributed in southern china.. with a few exceptions in vietnam. it is a cherished element used in traditional chinese medicine. in order to produce a global panorama of gene expression in the agkistrodon acutus venom gland, a non-normalized cdna library was constructed,. and 8696 high quality 5' end expressed sequenced tags (ests) were sequenced and analyzed. the initial sequences were assembled into 2855 clusters. of these clusters, only 45.60% clusters matched known sequence and 54.40% had no match to any known sequence in genbank. except for putative cellular proteins (1184 clusters), the remaining 118 clusters (40.16% of all ests) corresponded to sequences associated with diverse toxin function. according to expression abundance, the major toxin components were metalloproteinases (32.08%) and c-type lectin (5.22%), and other components including bradykinin-potentiating peptide (0.90%), serine proteases (0.51%), nucleotidase and nuclease (0.41%), phospholipase a2 (0.30%), disintegrin (0.05%), cytokine-like molecules (0.06%), and other proteins (0.63%). the majority of these components are thought to be responsible for coagulopathy after a. acutus bites. we have therefore generated a comprehensive catalog of the a. acutus venom gland described so far. gene expression from the very specialized secretory tissue, especially for those involved in coagulopathy, can be surveyed and provide important information in finding novel toxins. (c) 2006 elsevier inc. all rights reserved.
WOS关键词	LECTIN-LIKE PROTEINS ; SNAKE-VENOM ; ACCELERATED EVOLUTION ; SERINE PROTEINASES ; PURIFICATION ; EXPRESSION ; CLONING ; CRYSTALLIZATION ; THROMBOSIS ; DIVERSITY
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics
WOS类目	Biochemistry & Molecular Biology ; Biophysics
语种	英语
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
WOS记录号	WOS:000235414400036
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2378772
专题	中国科学院大学
通讯作者	Yan, GM
作者单位	1.Sun Yat Sen Univ, Zhong Shan Med Coll, Dept Pharmacol, Guangzhou 510089, Peoples R China 2.Chinese Acad Sci, Beijing Genom Inst, Beijing 101300, Peoples R China 3.Sun Yat Sen Univ, Zhong Shan Med Coll, Dept Biochem, Guangzhou 510089, Peoples R China 4.Grad Univ, Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Liu, QH,Zhang, XW,Wei, Y,et al. A catalog for transcripts in the venom gland of the agkistrodon acutus: identification of the toxins potentially involved in coagulopathy[J]. Biochemical and biophysical research communications,2006,341(2):522-531.
APA	Liu, QH.,Zhang, XW.,Wei, Y.,Li, CJ.,Huang, YJ.,...&Yan, GM.(2006).A catalog for transcripts in the venom gland of the agkistrodon acutus: identification of the toxins potentially involved in coagulopathy.Biochemical and biophysical research communications,341(2),522-531.
MLA	Liu, QH,et al."A catalog for transcripts in the venom gland of the agkistrodon acutus: identification of the toxins potentially involved in coagulopathy".Biochemical and biophysical research communications 341.2(2006):522-531.

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