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	Overexpression of the saussurea medusa chalcone isomerase gene in saussurea involucrata hairy root cultures enhances their biosynthesis of apigenin
	Li, FX; Jin, ZP; Zhao, DX; Cheng, LQ; Fu, CX; Ma, FS
刊名	Phytochemistry
	2006-03-01
卷号	67期号:6页码:553-560
关键词	Saussurea involucrata Compositae Hairy root Genetic transformation Chalcone isomerase Flavonoids Apigenin
ISSN号	0031-9422
DOI	10.1016/j.phytochem.2005.12.004
通讯作者	Zhao, dx()
英文摘要	Saussurea involucrata is a medicinal plant well known for its flavonoids, including apigenin, which has been shown to significantly inhibit tumorigenesis. since naturally occurring apigenin is in very low abundance, we took a transgenic approach to increase apigenin production by engineering the flavonoid pathway. a construct was made to contain the complete cdna sequence of the saussurea medusa chalcone isornerase (chi) gene under the control of the cauliflower mosaic virus (camv) 35s promoter. using an agrobacterium rhizogenes-mediated transformation system, the chi overexpression cassette was incorporated into the genome of s. involucrata, and transgenic hairy root lines were established. chi converts naringenin chalcone into naringenin that is the precursor of apigenin. we observed that transgenic hairy root lines grew faster and produced higher levels of apigenin and total flavonoids than wild-type hairy roots did. over a culture period of 5 weeks, the best-performing line (c46) accumulated 32.1 mg l-1 apigenin and 647.8 mg l-1 total flavonoids, or 12 and 4 times, respectively, higher than wild-type hairy roots did. the enhanced productivity corresponded to elevated chi activity, confirming the key role that chi played for total flavonoids and apigenin synthesis and the efficiency of the current metabolic engineering strategy. (c) 2005 elsevier ltd. all rights reserved.
WOS关键词	AGROBACTERIUM-RHIZOGENES ; ALKALOID PRODUCTION ; HYOSCYAMUS-MUTICUS ; PURIFICATION ; GROWTH ; TRANSFORMATION ; INHIBITION ; MECHANISM ; PLASMID ; BINDING
WOS研究方向	Biochemistry & Molecular Biology ; Plant Sciences
WOS类目	Biochemistry & Molecular Biology ; Plant Sciences
语种	英语
出版者	PERGAMON-ELSEVIER SCIENCE LTD
WOS记录号	WOS:000236163300005
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2378641
专题	中国科学院大学
通讯作者	Zhao, DX
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Photosynth & Environm Mol Physiol, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Univ Waterloo, Dept Biol, Waterloo, ON N2L 3G1, Canada
推荐引用方式 GB/T 7714	Li, FX,Jin, ZP,Zhao, DX,et al. Overexpression of the saussurea medusa chalcone isomerase gene in saussurea involucrata hairy root cultures enhances their biosynthesis of apigenin[J]. Phytochemistry,2006,67(6):553-560.
APA	Li, FX,Jin, ZP,Zhao, DX,Cheng, LQ,Fu, CX,&Ma, FS.(2006).Overexpression of the saussurea medusa chalcone isomerase gene in saussurea involucrata hairy root cultures enhances their biosynthesis of apigenin.Phytochemistry,67(6),553-560.
MLA	Li, FX,et al."Overexpression of the saussurea medusa chalcone isomerase gene in saussurea involucrata hairy root cultures enhances their biosynthesis of apigenin".Phytochemistry 67.6(2006):553-560.

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