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	Heat shock protein 27 downregulates the transferrin receptor 1-mediated iron uptake
	Chen, Huaiyong; Zheng, Chunlei; Zhang, Yong; Chang, Yan-Zhong; Qian, Zhong-Ming; Shen, Xun
刊名	International journal of biochemistry & cell biology
	2006
卷号	38期号:8页码:1402-1416
关键词	Heat shock protein 27 Transferrin receptor-mediated iron uptake Transferrin recycling Iron regulatory protein Actin polymerization
ISSN号	1357-2725
DOI	10.1016/j.biocel.2006.02.006
通讯作者	Qian, zhong-ming()
英文摘要	It has been reported that over-expression of human heat shock protein 27 (hsp27) in murine cells decreased the intracellular iron level [arrigo, a. p., virot, s., chaufour, s., firdaus, w., kretz-remy, c., & diaz-latoud, c. (2005). hsp27 consolidates intracellular redox homeostasis by upholding glutathione in its reduced form and by decreasing iron intracellular levels. antioxidants & redox signalling, 7, 412-422]. however, the mechanism involved is unknown. in this study, the regulation of transferrin receptor 1 (tfr1)-mediated iron uptake by human hsp27 was investigated in ccl39 cells by overexpression of human hsp27 and its dominant-negative mutant (hsp27-3g). the results showed that overexpression of hsp27 diminished intracellular labile iron pool, increased the binding activity of iron regulatory protein (irp) to iron responsive element (ire) and the cell surface-expressed tfr1s. however, the increased surface-expressed tfr1s resulted in decrease rather than increase of iron uptake. further study revealed that overexpression of hsp27 decelerated transferrin endocytosis and recycling, while overexpressed hsp27-3g had a reversal,effect. moreover, flowcytometric analysis showed an enhanced actin polymerization in the cells overexpressing hsp27. in particular, fluorescence imaging of cytoskeleton displayed highly stabilized microfilaments and preferential localization of hsp27 in cortical area of the actin cytoskeleton. in contrast, disruption of actin cytoskeleton by cytochalasin b resulted in acceleration of the endocytosis and recycling of tf, as well as increase of iron uptake. meanwhile, the possible involvement of ferroportin 1 in downregulation of intracellular iron level by overexpression of hsp27 was checked. however, the outcome was negative. our findings indicated that hsp27 down-regulates tfr1-mediated iron uptake via stabilization of the cortical actin cytoskeleton rather than the classical irp/ire mode. the study may also imply that hsp27 protects cells from oxidative stress by reducing cellular iron uptake. (c) 2006 elsevier ltd. all rights reserved.
WOS关键词	HEAT-SHOCK-PROTEIN ; HEREDITARY HEMOCHROMATOSIS PROTEIN ; ACTIN CYTOSKELETON ; RESPONSIVE ELEMENT ; OXIDATIVE STRESS ; MESSENGER-RNA ; 5-UNTRANSLATED REGION ; MEDIATED ENDOCYTOSIS ; KINASE CASCADE ; HSP 27
WOS研究方向	Biochemistry & Molecular Biology ; Cell Biology
WOS类目	Biochemistry & Molecular Biology ; Cell Biology
语种	英语
出版者	PERGAMON-ELSEVIER SCIENCE LTD
WOS记录号	WOS:000238822600017
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2378501
专题	中国科学院大学
通讯作者	Qian, Zhong-Ming
作者单位	1.Chinese Acad Sci, Grad Sch, Inst Biophys, Beijing 100101, Peoples R China 2.Hebei Normal Univ, Inst Mol Neurobiol & Neuropharmacol, Shijiazhuang 050016, Peoples R China 3.Hong Kong Polytech Univ, Dept Appl Biol & Chem Technol, Kowloon, Hong Kong, Peoples R China
推荐引用方式 GB/T 7714	Chen, Huaiyong,Zheng, Chunlei,Zhang, Yong,et al. Heat shock protein 27 downregulates the transferrin receptor 1-mediated iron uptake[J]. International journal of biochemistry & cell biology,2006,38(8):1402-1416.
APA	Chen, Huaiyong,Zheng, Chunlei,Zhang, Yong,Chang, Yan-Zhong,Qian, Zhong-Ming,&Shen, Xun.(2006).Heat shock protein 27 downregulates the transferrin receptor 1-mediated iron uptake.International journal of biochemistry & cell biology,38(8),1402-1416.
MLA	Chen, Huaiyong,et al."Heat shock protein 27 downregulates the transferrin receptor 1-mediated iron uptake".International journal of biochemistry & cell biology 38.8(2006):1402-1416.

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