| Disposable electrochemical biosensor for amperometric measurement of beta-hydroxybutyrate and total cholesterol | |
| Li, Huaqing; Wang, Li; Rao, Nenggao; Cui, Dafu; Cai, Xinxia | |
| 刊名 | Rare metal materials and engineering
 |
| 2006-12-01 | |
| 卷号 | 35页码:305-308 |
| 关键词 | Amperometric Beta-hydroxybutyrate Total cholesterol Ferricyanide Platinum black |
| ISSN号 | 1002-185X |
| 通讯作者 | Cai, xinxia(xxcai@mail.ie.ac.cn) |
| 英文摘要 | A new type of disposable thin-film amperometric biosensor has been developed for measurement of beta-hydroxybutyrate and total cholesterol in serum. the biosensor consists of two plain gold electrodes mounted on a polyethylene terephthalate (pet) substrate. the reagent solution contains bienzymes (cholesterol oxidase and cholesterol esterase for total cholesterol, beta-hydroxybutyrate dehydrogenase and diaphorase for beta-hydroxybutyrate respectively) with mediator (ferricyanide) were absorption at the surface of electrodes coated by electrodepositing platinum black. the presence of the mediator lowers the applied potential and eliminates the interference from other oxidizable species enhancing the sensitivity and selectivity of the biosensor without modifying the dynamic parameters of the response. the enzymes stably retains in the matrices of platinum black film improving the performance of the mediated sensor. the linearity is observed in the concentration range from 10 x 10(-4) to 10 x 10(-2) mm and 10 x 10(-4) mm to 4.99 x 10(-3) mm with sensitivity of 1.958 mu a/mm and 2.447 mu a/mm respectively. the optimized biosensor exhibits excellent reproducibility and stability retaining more than 90% of its original activity over a period of one month. the simple operation of the biosensor mass-produced at low cost is expected to find clinical application and homecare. |
| WOS关键词 | GLUCOSE ; MICROELECTRODE ; DEVICE ; PERFORMANCE ; ELECTRODES ; SENSORS ; ARRAY |
| WOS研究方向 | Materials Science ; Metallurgy & Metallurgical Engineering |
| WOS类目 | Materials Science, Multidisciplinary ; Metallurgy & Metallurgical Engineering |
| 语种 | 英语 |
| 出版者 | NORTHWEST INST NONFERROUS METAL RESEARCH |
| WOS记录号 | WOS:000244457000097 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2378463 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Cai, Xinxia |
| 作者单位 | 1.Chinese Acad Sci, Inst Elect, State Key Lab Transducer Technol, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Huaqing,Wang, Li,Rao, Nenggao,et al. Disposable electrochemical biosensor for amperometric measurement of beta-hydroxybutyrate and total cholesterol[J]. Rare metal materials and engineering,2006,35:305-308. |
| APA | Li, Huaqing,Wang, Li,Rao, Nenggao,Cui, Dafu,&Cai, Xinxia.(2006).Disposable electrochemical biosensor for amperometric measurement of beta-hydroxybutyrate and total cholesterol.Rare metal materials and engineering,35,305-308. |
| MLA | Li, Huaqing,et al."Disposable electrochemical biosensor for amperometric measurement of beta-hydroxybutyrate and total cholesterol".Rare metal materials and engineering 35(2006):305-308. |
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