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	Effect of tri-o-cresyl phosphate on cytoskeleton in human neuroblastoma sk-n-sh cell
	Chang, Ping-An; Wu, Yi-Jun
刊名	Molecular and cellular biochemistry
	2006-10-01
卷号	290期号:1-2页码:145-151
关键词	Human neuroblastoma sk-n-sh cell Neurotoxicity Cytoskeleton Esterase Tri-o-cresyl phosphate
ISSN号	0300-8177
DOI	10.1007/s11010-006-9181-z
通讯作者	Wu, yi-jun(wuyj@ioz.ac.cn)
英文摘要	Cytoskeletal components play an important role in maintaining cellular architecture and internal organization, with clear involvement of defining cell shape, in cell division and other cellular processes, such as neurite extension and maintenance. alterations of cytoskeleton in human neuroblastoma sk-n-sh cells after exposure to different concentrations of tri-ocresyl phosphate (tocp) for 12 hr were investigated. tocp decreased the cell viability in a dose-dependent manner; the viability of sk-n-sh was reduced to approximately 50% of baseline after a 12-hour exposure to tocp at high concentration (5 mm). biochemical characterization by western blotting revealed that 1 and 5 mm concentrations of tocp significantly inhibited the expression of neurofilament high molecular weight protein (nf-h), and that 5 mm tocp inhibited expression of microtubule-associated protein 2c and tau protein, but not beta-actin. indirect immunofluorescence analysis revealed that higher concentrations of tocp decreased the length of neuritis and changed the structure of microfilaments, which are associated with nf-h. in addition, activities of neuropathy target esterase and acetylcholinesterase were significantly reduced after exposure to 5 mm tocp for 12 hr. together, these results suggested that the loss of cytoskeletal components is the early event during the process of tocp toxicity towards human neuroblastoma sk-n-sh cells.
WOS关键词	AXON-LIKE PROCESSES ; ORGANOPHOSPHORUS COMPOUNDS ; DELAYED NEUROTOXICITY ; TRICRESYL PHOSPHATE ; MOUSE N2A ; ESTERASE ; TARGET ; SENSITIVITY ; PROTEINS ; ASSAY
WOS研究方向	Cell Biology
WOS类目	Cell Biology
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000240802100018
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2378445
专题	中国科学院大学
通讯作者	Wu, Yi-Jun
作者单位	1.Chinese Acad Sci, Inst Zool, State Keyu Lab Integrated Management Pest Insects, Mol Toxicol Lab, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Chang, Ping-An,Wu, Yi-Jun. Effect of tri-o-cresyl phosphate on cytoskeleton in human neuroblastoma sk-n-sh cell[J]. Molecular and cellular biochemistry,2006,290(1-2):145-151.
APA	Chang, Ping-An,&Wu, Yi-Jun.(2006).Effect of tri-o-cresyl phosphate on cytoskeleton in human neuroblastoma sk-n-sh cell.Molecular and cellular biochemistry,290(1-2),145-151.
MLA	Chang, Ping-An,et al."Effect of tri-o-cresyl phosphate on cytoskeleton in human neuroblastoma sk-n-sh cell".Molecular and cellular biochemistry 290.1-2(2006):145-151.

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