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	A novel matrix for high performance affinity chromatography and its application in the purification of antithrombin iii
	Zhao, R; Luo, H; Shangguan, D; Liu, GQ
刊名	Journal of chromatography b-analytical technologies in the biomedical and life sciences
	2005-02-25
卷号	816期号:1-2页码:175-181
关键词	Viscose fiber High performance affinity chromatography Heparin Antithrombin iii
ISSN号	1570-0232
DOI	10.1016/j.jchromb.2004.11.030
通讯作者	Zhao, r(zhaorui@iccas.ac.cn)
英文摘要	Viscose fiber, a regenerated cellulose, was evaluated for using as a novel matrix for high performance affinity chromatography. with a one-step activation with epichlorohydrin, heparin can be readily covalently attached to the matrix. this heparin-viscose fiber material was used for purifying antithrombin iii (at iii) from human plasma. the purity of the at iii from this one-step purification is 93% as measured by sds-page and the protein recovery yield is about 90%. this column is highly specific as described by the dissociation constant of the complex of immobilized heparin and at iii, which was 2.83 x 10(-5) mol/l. and more important, this viscose fiber material demonstrated its excellent mechanical property that allows the flow rate to reach up to 900 cm/h or more. (c) 2004 elsevier b.v. all rights reserved.
WOS关键词	CONCANAVALIN-A ; BINDING ; PROTEINS ; HEPARIN ; RECOGNITION ; SEPARATION ; CELLULOSE ; PEPTIDES ; SUPPORTS ; LIGANDS
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000226879800023
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2378366
专题	中国科学院大学
通讯作者	Zhao, R
作者单位	1.Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Zhao, R,Luo, H,Shangguan, D,et al. A novel matrix for high performance affinity chromatography and its application in the purification of antithrombin iii[J]. Journal of chromatography b-analytical technologies in the biomedical and life sciences,2005,816(1-2):175-181.
APA	Zhao, R,Luo, H,Shangguan, D,&Liu, GQ.(2005).A novel matrix for high performance affinity chromatography and its application in the purification of antithrombin iii.Journal of chromatography b-analytical technologies in the biomedical and life sciences,816(1-2),175-181.
MLA	Zhao, R,et al."A novel matrix for high performance affinity chromatography and its application in the purification of antithrombin iii".Journal of chromatography b-analytical technologies in the biomedical and life sciences 816.1-2(2005):175-181.

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