Inhibitory effect of epimedium extract on s-adenosyl-l-homocysteine hydrolase and biomethylation | |
Zhang, XY; Li, YJ; Yang, XD; Wang, K; Ni, JZ; Qu, XG | |
刊名 | Life sciences |
2005-11-26 | |
卷号 | 78期号:2页码:180-186 |
关键词 | Epimedium extract S-adenosyl-l-homocysteine hydrolase Biomethylation |
ISSN号 | 0024-3205 |
DOI | 10.1016/j.lfs.2005.04.057 |
通讯作者 | Ni, jz() |
英文摘要 | In the present paper, the inhibitory effect of epimedium extract on the activity of s-adenosyl-l-homocysteine (adohcy) hydrolase was studied. the results showed that epimedium extract inhibited the activity of recombinant human adohcy hydrolase in a dose-dependent manner. this inhibitory effect was also observed in hepatic cell line 7701 and hepatoma hepg2, however, the effect in 7701 cells was more potent than in hepg2 cells. the extract could significantly reduce adomet/adohcy ratio in 7701 cells in a dose-dependent manner, suggesting reduced biomethylation level in 7701 cells. in contrast, it resulted in elevated adomet/adohcy ratio in the hepg2 cells. the result of maldi-ms assay indicated that epimedin a and ikarisoside f from the extract could bind to adohcy hydrolase. the present data suggested that epimedium extract could inhibit the activity of adohcy hydrolase, thus regulating the cellular biomethylation as well as reducing cellular hcy level. these results will provide new clues to the mechanisms of epimedium in curing of cardiovascular disease and regulating tumor cell growth. (c) 2005 elsevier inc. all rights reserved. |
WOS关键词 | ADENOSYLHOMOCYSTEINE HYDROLASE ; TRANSPORT ACTIVITY ; HUMAN-FIBROBLASTS ; ADENOSYLMETHIONINE ; TRANSMETHYLATIONS ; INDUCTION ; DISEASE ; CYSTINE ; BINDING ; FOLATE |
WOS研究方向 | Research & Experimental Medicine ; Pharmacology & Pharmacy |
WOS类目 | Medicine, Research & Experimental ; Pharmacology & Pharmacy |
语种 | 英语 |
出版者 | PERGAMON-ELSEVIER SCIENCE LTD |
WOS记录号 | WOS:000233187400009 |
内容类型 | 期刊论文 |
URI标识 | http://www.corc.org.cn/handle/1471x/2378334 |
专题 | 中国科学院大学 |
通讯作者 | Ni, JZ |
作者单位 | 1.Chinese Acad Sci, Grad Sch, Changchun Inst Appl Chem, Changchun 130022, Peoples R China 2.Peking Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, Dept Biol Chem, Beijing 100083, Peoples R China 3.Peking Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, Natl Res Labs Nat & Biomimet Drugs, Beijing 100083, Peoples R China |
推荐引用方式 GB/T 7714 | Zhang, XY,Li, YJ,Yang, XD,et al. Inhibitory effect of epimedium extract on s-adenosyl-l-homocysteine hydrolase and biomethylation[J]. Life sciences,2005,78(2):180-186. |
APA | Zhang, XY,Li, YJ,Yang, XD,Wang, K,Ni, JZ,&Qu, XG.(2005).Inhibitory effect of epimedium extract on s-adenosyl-l-homocysteine hydrolase and biomethylation.Life sciences,78(2),180-186. |
MLA | Zhang, XY,et al."Inhibitory effect of epimedium extract on s-adenosyl-l-homocysteine hydrolase and biomethylation".Life sciences 78.2(2005):180-186. |
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