QQ客服 官方微博 反馈留言

	H2o2 directly activates inositol 1,4,5-trisphosphate receptors in endothelial cells
	Zheng, Y; Shen, X
刊名	Redox report
	2005
卷号	10期号:1页码:29-36
关键词	Hydrogen peroxide Ip3 receptors Endothelial cells Ca2+ mobilization
ISSN号	1351-0002
DOI	10.1179/135100005x21660
通讯作者	Shen, x()
英文摘要	The mechanisms of h2o2-induced ca2+ release from intracellular stores were investigated in human umbilical vein endothelial cells. it was found that u73122, the selective inhibitor of phospholipase c, could not inhibit the h2o2-induced cytosolic ca2+ mobilization. no elevation of inositol 1,4,5-trisphosphate (ip3) was detected in cells exposed to h2o2. by loading mag-fura-2, a ca2+ indicator, into intracellular store, the h2o2-induced ca2+ release from intracellular calcium store was directly observed in the permeabilized cells in a dose-dependent manner. this release can be completely blocked by heparin, a well-known antagonist of ip3 receptor, indicating a direct activation of ip3 receptor on endoplasmic reticulum (er) membrane by h2o2. it was also found that h2o2 could still induce a relatively small ca2+ release from internal stores after the ca2+-atpase on er membrane and the ca2+ uptake to mitochondria were simultaneously inhibited by thapsigargin and carbonyl cyanide p-trifluoromethoxyphenyl hydrazone. the later observation suggests that a thapsigargin-insensitive non-mitochondrial intracellular ca2+ store might be also involved in h2o2-induced ca2+ mobilization.
WOS关键词	SMOOTH-MUSCLE-CELLS ; PLASMA-MEMBRANE CA2+-ATPASE ; HYDROGEN-PEROXIDE ; SARCOPLASMIC-RETICULUM ; ENDOPLASMIC-RETICULUM ; PHOSPHOLIPASE-C ; CALCIUM-ENTRY ; MITOCHONDRIAL ; RELEASE ; CHANNEL
WOS研究方向	Biochemistry & Molecular Biology
WOS类目	Biochemistry & Molecular Biology
语种	英语
出版者	MANEY PUBLISHING
WOS记录号	WOS:000227829100005
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2377877
专题	中国科学院大学
通讯作者	Shen, X
作者单位	Chinese Acad Sci, Inst Biophys, Grad Sch, Beijing 100101, Peoples R China
推荐引用方式 GB/T 7714	Zheng, Y,Shen, X. H2o2 directly activates inositol 1,4,5-trisphosphate receptors in endothelial cells[J]. Redox report,2005,10(1):29-36.
APA	Zheng, Y,&Shen, X.(2005).H2o2 directly activates inositol 1,4,5-trisphosphate receptors in endothelial cells.Redox report,10(1),29-36.
MLA	Zheng, Y,et al."H2o2 directly activates inositol 1,4,5-trisphosphate receptors in endothelial cells".Redox report 10.1(2005):29-36.

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。