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	Human protein tau represses dna replication in vitro
	Li, W; Wang, XS; Qu, MH; Liu, Y; He, RQ
刊名	Biochimica et biophysica acta-general subjects
	2005-11-30
卷号	1726期号:3页码:280-286
关键词	Protein tau Dna replication Transcription Repression Pcr
ISSN号	0304-4165
DOI	10.1016/j.bbagen.2005.08.014
通讯作者	He, rq()
英文摘要	Here, in the experiments of both pcr and real-time pcr, a repression of dna amplification was observed in the presence of protein tau. furthermore, a strong repression appeared when an in vitro dna replication assay was performed at the physiological temperature (37 degrees c). the incorporation of dntp was markedly decreased to similar to 12% of control by the presence of tau23 and to similar to 15% by tau40. in the competitive experiments, the pcr product could be restored when the competitor dna was added, indicating that the association of tau with the template gave rise to the repression. however, tau did not repress the yield of rna in transcription, suggesting that tau was replaced or ejected from the template by the elongating t7 rna polymerase. (c) 2005 elsevier b.v. all rights reserved.
WOS关键词	MICROTUBULE-ASSOCIATED PROTEIN ; SINGLE-STRANDED-DNA ; CELLS ; PHOSPHORYLATION ; POLYMERASE ; NUCLEOSOME ; MICROSCOPY ; NUCLEAR ; BINDS ; ASSAY
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics
WOS类目	Biochemistry & Molecular Biology ; Biophysics
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000233709400007
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2377873
专题	中国科学院大学
通讯作者	He, RQ
作者单位	1.Chinese Acad Sci, Inst Biophys, State Key Lab Brain & Cognit Sci, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Li, W,Wang, XS,Qu, MH,et al. Human protein tau represses dna replication in vitro[J]. Biochimica et biophysica acta-general subjects,2005,1726(3):280-286.
APA	Li, W,Wang, XS,Qu, MH,Liu, Y,&He, RQ.(2005).Human protein tau represses dna replication in vitro.Biochimica et biophysica acta-general subjects,1726(3),280-286.
MLA	Li, W,et al."Human protein tau represses dna replication in vitro".Biochimica et biophysica acta-general subjects 1726.3(2005):280-286.

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