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	Continuous on-line monitoring of extracellular ascorbate depletion in the rat striatum induced by global ischemia with carbon nanotube-modified glassy carbon electrode integrated into a thin-layer radial flow cell
	Zhang, MN; Liu, K; Gong, KP; Su, L; Chen, Y; Mao, LQ
刊名	Analytical chemistry
	2005-10-01
卷号	77期号:19页码:6234-6242
ISSN号	0003-2700
DOI	10.1021/ac051188d
通讯作者	Mao, lq()
英文摘要	This study describes a novel analytical system integrating in vivo microdialysis sampling with a radial thin-layer flow cell with a single-walled carbon nanotube (swnt)-modified glassy carbon electrode as working electrode for continuous and on-line monitoring of ascorbate depletion in the rat striatum induced by global ischemia. the swnts, especially those after vacuum heat treatment at 500 degrees c, are found to be able to enhance the electron-transfer kinetics of ascorbate oxidation at a low potential (ca. -50 mv) and possess a strong ability against electrode fouling. these properties essentially make it possible to determine ascorbate with a good stability and high selectivity against catecholamines and their metabolites and other electroactive species of physiological levels. while being integrated with in vivo microdialysis to assemble an on-line analytical system, the electrode is proved useful for continuous and sensitive monitoring of the basal dialysate level of ascorbate and its depletion in the rat striatum induced by global ischemia. the basal dialysate level of ascorbate is determined to be 5.0 +/- 0.5 mu m (n = 5) and a 50 +/- 10% (n = 3) depletion is recorded for the basal ascorbate after 4 h of global ischemia.
WOS关键词	FREELY MOVING RATS ; SELF-ASSEMBLED MONOLAYER ; CENTRAL-NERVOUS-SYSTEM ; CEREBRAL-ISCHEMIA ; CYTOCHROME-C ; ELECTROCHEMICAL DETECTION ; SUPEROXIDE-DISMUTASE ; ACID RELEASE ; BEHAVIORAL ACTIVATION ; MERCURY-ELECTRODE
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000232443700030
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2377844
专题	中国科学院大学
通讯作者	Mao, LQ
作者单位	1.Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Zhang, MN,Liu, K,Gong, KP,et al. Continuous on-line monitoring of extracellular ascorbate depletion in the rat striatum induced by global ischemia with carbon nanotube-modified glassy carbon electrode integrated into a thin-layer radial flow cell[J]. Analytical chemistry,2005,77(19):6234-6242.
APA	Zhang, MN,Liu, K,Gong, KP,Su, L,Chen, Y,&Mao, LQ.(2005).Continuous on-line monitoring of extracellular ascorbate depletion in the rat striatum induced by global ischemia with carbon nanotube-modified glassy carbon electrode integrated into a thin-layer radial flow cell.Analytical chemistry,77(19),6234-6242.
MLA	Zhang, MN,et al."Continuous on-line monitoring of extracellular ascorbate depletion in the rat striatum induced by global ischemia with carbon nanotube-modified glassy carbon electrode integrated into a thin-layer radial flow cell".Analytical chemistry 77.19(2005):6234-6242.

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