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	Arabidopsis soi33/atent8 gene encodes a putative equilibrative nucleoside transporter that is involved in cytokinin transport in planta
	Sun, JP; Hirose, N; Wang, XC; Wen, P; Xue, L; Sakakibara, H; Zuo, JR
刊名	Journal of integrative plant biology
	2005-05-01
卷号	47期号:5页码:588-603
关键词	Arabidopsis Cytokinin transport Equilibrative nucleoside transporter Soi33
ISSN号	1672-9072
通讯作者	Zuo, jr()
英文摘要	The plant phytohormone cytokinin plays an important role in many facets of plant growth and development by regulating cell division and differentiation. recent studies have shed significant light into the mechanisms of cytokinin metabolism and signaling. however, little is known about how the hormone is transported in planta, although it has been proposed that the hormone is presumably transported in nucleoside-conjugated forms. here, we report the identification and characterization of cytokinin transporters in arabidopsis. we previously reported that a gain-of-function mutation in the pga22/atipt8 gene caused overproduction of cytokinins in planta. in an effort to screen for suppressor of pga22/atipt8 (soi) mutants, we identified a mutant soi33-1. molecular and genetic analyses indicated that soi33 encodes a putative equilibrative nucleoside transporter (ent), previously designated as atent8. members of this small gene family are presumed to be involved in the transport of nucleosides in eukaryotic cells. under conditions of nitrogen starvation, loss-of-function mutations in soi33/atent8 or in a related gene atent3 cause a reduced sensitivity to the nucleoside-type cytokinins isopentenyladenine riboside (ipr) and transzeatin riboside (tzr), but display a normal response to the free base-type cytokinins isopentenyladenine (ip) and trans-zeatin (tz). conversely, overexpression of soi33/atent8 renders transgenic plants hypersensitive to ipr but not to w. an in planta measurement experiment indicated that uptake efficiency of h-3-labeled ipr was reduced more than 40% in soi33 and atent3 mutants. however, a mutation in atent1 had no substantial effect on the cytokinin response and ipr uptake efficiency. our results suggest that soi33/ atent8 and atent3 are involved in the transport of nucleoside-type cytokinins in arabidopsis.
WOS关键词	SIGNAL-TRANSDUCTION ; RESPONSE REGULATORS ; HISTIDINE KINASE ; AUXIN TRANSPORT ; ENT FAMILY ; THALIANA ; BIOSYNTHESIS ; IDENTIFICATION ; EXPRESSION ; RECEPTOR
WOS研究方向	Biochemistry & Molecular Biology ; Plant Sciences
WOS类目	Biochemistry & Molecular Biology ; Plant Sciences
语种	英语
出版者	BLACKWELL PUBLISHING ASIA
WOS记录号	WOS:000229336000009
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2377598
专题	中国科学院大学
通讯作者	Zuo, JR
作者单位	1.Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.RIKEN, Plant Sci Ctr, Yokohama, Kanagawa 2300045, Japan
推荐引用方式 GB/T 7714	Sun, JP,Hirose, N,Wang, XC,et al. Arabidopsis soi33/atent8 gene encodes a putative equilibrative nucleoside transporter that is involved in cytokinin transport in planta[J]. Journal of integrative plant biology,2005,47(5):588-603.
APA	Sun, JP.,Hirose, N.,Wang, XC.,Wen, P.,Xue, L.,...&Zuo, JR.(2005).Arabidopsis soi33/atent8 gene encodes a putative equilibrative nucleoside transporter that is involved in cytokinin transport in planta.Journal of integrative plant biology,47(5),588-603.
MLA	Sun, JP,et al."Arabidopsis soi33/atent8 gene encodes a putative equilibrative nucleoside transporter that is involved in cytokinin transport in planta".Journal of integrative plant biology 47.5(2005):588-603.

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