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	Lysosome destabilization by cytosolic extracts, putative involvement of ca2+/phospholipase c
	Zhao, HF; Wang, X; Zhang, GH
刊名	Febs letters
	2005-02-28
卷号	579期号:6页码:1551-1556
关键词	Lysosome Phospholipase c Osmotic sensitivity Calcium ion
ISSN号	0014-5793
DOI	10.1016/j.febslet.2005.01.061
通讯作者	Zhang, gh()
英文摘要	Lysosomal disintegration is a crucial event for living cells, but mechanisms for the event are still unclear. in this study, we established that the cytosolic extracts could enhance lysosomal osmotic sensitivity and osmotically destabilize the lysosomes. the cytosol also caused the lysosomes to become more swollen in the hypotonic sucrose medium. the results indicate that the cytosol induced an osmotic shock to the lysosomes and an influx of water into the organelle. since the effects of cytosol on the lysosomes could be abolished by o-tricyclo[5.2. 1.0(2,6)]dec-9-yl dithiocarbonate potassium salt (d609), a specific inhibitor of cytosolic phospholipase c (plc), the plc might play an important role in the lysosomal osmotic destabilization. the activity of cytosolic plc and the extent of enzyme latency loss of the cytosol-treated lysosomes exhibited a similar biphasic dependence on the cytosolic ca2+ concentration. in addition, the cytosol did not osmotically destabilize the lysosomes until the cytosolic calcium ions rose above 100 nm. it suggests that the destabilization effect of cytosol on the lysosomes is ca2+-dependent. (c) 2005 federation of european biochemical societies. published by elsevier b.v. all rights reserved.
WOS关键词	RAT-LIVER LYSOSOMES ; PHOSPHOLIPASE-C ; CELL-DEATH ; OSMOTIC SENSITIVITY ; CALCIUM ; APOPTOSIS ; MEMBRANE ; TRANSLOCATION ; ATP
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
WOS类目	Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000227393700038
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2377475
专题	中国科学院大学
通讯作者	Zhang, GH
作者单位	1.Chinese Acad Sci, Inst Biophys, Beijing 100101, Peoples R China 2.Univ Sci & Technol Beijing, Beijing 100083, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100101, Peoples R China
推荐引用方式 GB/T 7714	Zhao, HF,Wang, X,Zhang, GH. Lysosome destabilization by cytosolic extracts, putative involvement of ca2+/phospholipase c[J]. Febs letters,2005,579(6):1551-1556.
APA	Zhao, HF,Wang, X,&Zhang, GH.(2005).Lysosome destabilization by cytosolic extracts, putative involvement of ca2+/phospholipase c.Febs letters,579(6),1551-1556.
MLA	Zhao, HF,et al."Lysosome destabilization by cytosolic extracts, putative involvement of ca2+/phospholipase c".Febs letters 579.6(2005):1551-1556.

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