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	Strontium promotes calcium oscillations in mouse meiotic oocytes and early embryos through insp3 receptors, and requires activation of phospholipase and the synergistic action of insp3
	Zhang, D; Pan, L; Yang, LH; He, XK; Huang, XY; Sun, FZ
刊名	Human reproduction
	2005-11-01
卷号	20期号:11页码:3053-3061
关键词	Calcium oscillations Embryos Insp3r Oocytes Sr2+
ISSN号	0268-1161
DOI	10.1093/humrep/dei215
通讯作者	Sun, fz()
英文摘要	Background: sr2+ is the most efficient agent for mouse oocyte activation and functions by inducing ca2+ oscillations. however, its specific mechanism of action remains unknown. here we investigated the specificity and possible mechanism of sr2+-induced ca2+ oscillations in mouse oocytes and early embryos. methods: ca2+ oscillations in oocytes and embryos were measured by ratiometric fluorescence imaging using fura-2am. the role of phospholipase c (plc) and inositol trisphosphate (insp3) receptors in sr2+-induced ca2+ oscillations was examined by selective inhibitors. results: sr2+ can induce ca2+ oscillations in both immature and mature oocytes, and in early embryos. a cell cycle stage-dependent phenomenon to sr2+ stimulation was observed in 1-cell embryos. by using a low molecular weight heparin to antagonize the function of insp3 receptors, we were able to show that insp3 receptors are essential for sr2+-induced ca2+ oscillations. treating metaphase ii (mii) oocytes with the plc inhibitor, u73122, abolished sr2+-induced increases in ca2+. this inhibitory effect of u73122 could be rescued by microinjection of insp3, indicating that sr2+-induced ca2+ oscillations require the synergistic action of insp3. conclusions: sr2+-induced calcium oscillations in mouse oocytes and early embryos are mediated through insp3 receptors, and require plc activation and the synergistic action of insp3.
WOS研究方向	Obstetrics & Gynecology ; Reproductive Biology
WOS类目	Obstetrics & Gynecology ; Reproductive Biology
语种	英语
出版者	OXFORD UNIV PRESS
WOS记录号	WOS:000233045700013
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2377244
专题	中国科学院大学
通讯作者	Sun, FZ
作者单位	1.Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100080, Peoples R China
推荐引用方式 GB/T 7714	Zhang, D,Pan, L,Yang, LH,et al. Strontium promotes calcium oscillations in mouse meiotic oocytes and early embryos through insp3 receptors, and requires activation of phospholipase and the synergistic action of insp3[J]. Human reproduction,2005,20(11):3053-3061.
APA	Zhang, D,Pan, L,Yang, LH,He, XK,Huang, XY,&Sun, FZ.(2005).Strontium promotes calcium oscillations in mouse meiotic oocytes and early embryos through insp3 receptors, and requires activation of phospholipase and the synergistic action of insp3.Human reproduction,20(11),3053-3061.
MLA	Zhang, D,et al."Strontium promotes calcium oscillations in mouse meiotic oocytes and early embryos through insp3 receptors, and requires activation of phospholipase and the synergistic action of insp3".Human reproduction 20.11(2005):3053-3061.

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