| Whole exon 5 and intron 5 replaced by rhce in d-va(hus) | |
| Shao, CP; Xiong, W; Wang, W | |
| 刊名 | Journal of human genetics
 |
| 2004-02-01 | |
| 卷号 | 49期号:2页码:106-108 |
| 关键词 | Rhd Rhce D-va D-vi Sequencing chinese |
| ISSN号 | 1435-232X |
| DOI | 10.1007/s10038-003-0112-5 |
| 通讯作者 | Shao, cp(cpshaos@hotmail.com) |
| 英文摘要 | The d-va(hus) was previously investigated through cdna analysis, which revealed an rhd-ce(5)-d hybrid allele. however, the 5' and 3' breakpoints remain unknown. in this article, gene recombinations between the rhd and rhce alleles were investigated by a combination approach of a sequence-specific primer pcr (pcr-ssp) and an rhd full-length coding region sequencing method on two chinese subjects with weak d phenotypes. the hybrid rhesus box of each individual was also investigated through an established pcr-based method. as a result, two partial d phenotypes, d-va(hus) and d-vi type iii, were identified, each carrying one hybrid rhd-ce-d allele. the two samples were also serotyped with rh phontypes of dccee and dccee, respectively. other sequencing analyses of the d-va(hus) sample showed that the sequence of intron 4 is identical with rhd, whereas the whole sequence of exon 5 and intron 5 is identical with rhce except for seven polymorphisms in the intron 5. we may concluded that in the case of this chinese d-va(hus), the whole exon 5 and complete intron 5 of a total segment of 1801 nucleotides were replaced by rhce suggesting that the breakpoints of the replaced region are the 5' end of the exon 5 and the 3' end of the intron 5. |
| WOS关键词 | MONOCLONAL ANTI-D ; D-VA ; PHENOTYPES ; EPITOPES ; CHINESE ; CELLS |
| WOS研究方向 | Genetics & Heredity |
| WOS类目 | Genetics & Heredity |
| 语种 | 英语 |
| 出版者 | SPRINGER-VERLAG TOKYO |
| WOS记录号 | WOS:000189284900008 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2376804 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Shao, CP |
| 作者单位 | 1.Shenzhen Blood Ctr, Futian 518035, Shenzhen, Peoples R China 2.Shenzhen Inst Transfus Med, Futian 518035, Shenzhen, Peoples R China 3.Chinese Acad Sci, Grad Sch, Dept Biol, Beijing, Peoples R China 4.Hong Kong Univ Sci & Technol, Peking Univ, Med Ctr, Shenzhen, Peoples R China 5.Peking Univ, Shenzhen Hosp, Shenzhen, Peoples R China 6.Peking Univ, Hlth Sci Ctr, Sch Publ Hlth, Beijing 100871, Peoples R China 7.Edith Cowan Univ, Ctr Human Genet, Perth, WA, Australia |
| 推荐引用方式 GB/T 7714 | Shao, CP,Xiong, W,Wang, W. Whole exon 5 and intron 5 replaced by rhce in d-va(hus)[J]. Journal of human genetics,2004,49(2):106-108. |
| APA | Shao, CP,Xiong, W,&Wang, W.(2004).Whole exon 5 and intron 5 replaced by rhce in d-va(hus).Journal of human genetics,49(2),106-108. |
| MLA | Shao, CP,et al."Whole exon 5 and intron 5 replaced by rhce in d-va(hus)".Journal of human genetics 49.2(2004):106-108. |
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