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	Enzyme activity highlights the importance of the oxidative pentose phosphate pathway in lipid accumulation and growth of phaeodactylum tricornutum under co2 concentration
	Wu, Songcui1,2; Huang, Aiyou1; Zhang, Baoyu1; Huan, Li1,2; Zhao, Peipei1,2; Lin, Apeng1; Wang, Guangce1
刊名	Biotechnology for biofuels
	2015-05-28
卷号	8页码:11
关键词	Co2 Lipids Enzyme activity Oxidative pentose phosphate pathway Phaeodactylum tricornutum
ISSN号	1754-6834
DOI	10.1186/s13068-015-0262-7
通讯作者	Wang, guangce(gcwang@qdio.ac.cn)
英文摘要	Background: rising co2 concentration was reported to increase phytoplankton growth rate as well as lipid productivity. this has raised questions regarding the nadph supply for high lipid synthesis as well as rapid growth of algal cells. results: in this study, growth, lipid content, photosynthetic performance, the activity, and expression of key enzymes in calvin cycle and oxidative pentose phosphate pathway (oppp) were analyzed in the marine diatom phaeodactylum tricornutum under three different co2 concentrations (low co2 (0.015 %), mid co2 (atmospheric, 0.035 %) and high co2 (0.15 %)). both the growth rate and lipid content of p. tricornutum increased significantly under the high co2 concentration. enzyme activity and mrna expression of three calvin cycle-related enzymes (rubisco, 3-phosphoglyceric phosphokinase (pgk), phosphoribulokinase (prk)) were also increased under high co2 cultivation, which suggested the enhancement of calvin cycle activity. this may account for the observed rapid growth rate. in addition, high activity and mrna expression of g6pdh and 6pgdh, which produce nadph through oppp, were observed in high co2 cultured cells. these results indicate oppp was enhanced and might play an important role in lipid synthesis under high co2 concentration. conclusions: the oxidative pentose phosphate pathway may participate in the lipid accumulation in rapid-growth p. tricornutum cells in high co2 concentration.
WOS关键词	ELEVATED CARBON-DIOXIDE ; NATURAL PHYTOPLANKTON ; ATMOSPHERIC CO2 ; MARINE DIATOM ; MICROALGAE ; ALGAE ; PHOTOSYNTHESIS ; METABOLISM ; PROTEIN ; ELECTROPHORESIS
WOS研究方向	Biotechnology & Applied Microbiology ; Energy & Fuels
WOS类目	Biotechnology & Applied Microbiology ; Energy & Fuels
语种	英语
出版者	BIOMED CENTRAL LTD
WOS记录号	WOS:000355966100001
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2376670
专题	中国科学院大学
通讯作者	Wang, Guangce
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Coll Earth Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wu, Songcui,Huang, Aiyou,Zhang, Baoyu,et al. Enzyme activity highlights the importance of the oxidative pentose phosphate pathway in lipid accumulation and growth of phaeodactylum tricornutum under co2 concentration[J]. Biotechnology for biofuels,2015,8:11.
APA	Wu, Songcui.,Huang, Aiyou.,Zhang, Baoyu.,Huan, Li.,Zhao, Peipei.,...&Wang, Guangce.(2015).Enzyme activity highlights the importance of the oxidative pentose phosphate pathway in lipid accumulation and growth of phaeodactylum tricornutum under co2 concentration.Biotechnology for biofuels,8,11.
MLA	Wu, Songcui,et al."Enzyme activity highlights the importance of the oxidative pentose phosphate pathway in lipid accumulation and growth of phaeodactylum tricornutum under co2 concentration".Biotechnology for biofuels 8(2015):11.

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