QQ客服 官方微博 反馈留言

	New insights into the posttranslational regulation of human cytosolic thioredoxin by s-palmitoylation
	Xu, Zhiyu; Zhong, Liangwei
刊名	Biochemical and biophysical research communications
	2015-05-15
卷号	460期号:4页码:949-956
关键词	Posttranslational modification Thioredoxin Ptp1b S-palmitoylation Insulin
ISSN号	0006-291X
DOI	10.1016/j.bbrc.2015.03.132
通讯作者	Zhong, liangwei(liazho@ucas.ac.cn)
英文摘要	High level of palmitate is associated with metabolic disorders. we recently showed that enhanced level of s-palmitoylated cytosolic thioredoxin (trx1) in mouse liver was new characteristic feature of insulin resistance. however, our understanding of the effect of s-palmitoylation on trx1 is limited, and the tissue specificity of trx1 s-palmitoylation is unclear. here we show that s-palmitoylation also occurs at cys73 of trx1 in living endothelial cells, and the level of s-palmitoylated trx1 undergoes regulation by insulin signaling. trx1 prefers thiol-thioester exchange with palmitoyl-coa to acetyl-coa. s-palmitoylation alters conformation or secondary structure of trx1, as well as decreases the ability of trx1 to transfer electrons from thioredoxin reductase to s-nitrosylated protein tyrosine phosphatase 1b and s-nitrosoglutathione. our results demonstrate that s-palmitoylation is an important post-translational modification of human trx1. (c) 2015 elsevier inc. all rights reserved.
WOS关键词	FATTY-ACID SYNTHASE ; INSULIN-RESISTANCE ; OXIDIZED PTP1B ; PROTEIN ; REDUCTASE ; NITROSYLATION ; FLUORESCENCE ; GLUTATHIONE ; MECHANISM ; APOPTOSIS
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics
WOS类目	Biochemistry & Molecular Biology ; Biophysics
语种	英语
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
WOS记录号	WOS:000356980900010
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2376416
专题	中国科学院大学
通讯作者	Zhong, Liangwei
作者单位	Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Xu, Zhiyu,Zhong, Liangwei. New insights into the posttranslational regulation of human cytosolic thioredoxin by s-palmitoylation[J]. Biochemical and biophysical research communications,2015,460(4):949-956.
APA	Xu, Zhiyu,&Zhong, Liangwei.(2015).New insights into the posttranslational regulation of human cytosolic thioredoxin by s-palmitoylation.Biochemical and biophysical research communications,460(4),949-956.
MLA	Xu, Zhiyu,et al."New insights into the posttranslational regulation of human cytosolic thioredoxin by s-palmitoylation".Biochemical and biophysical research communications 460.4(2015):949-956.

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。