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De novo transcriptome analysis reveals distinct defense mechanisms by young and mature leaves of hevea brasiliensis (para rubber tree)
Fang, Yongjun1; Mei, Hailiang2,3; Zhou, Binhui1; Xiao, Xiaohu1; Yang, Meng2; Huang, Yacheng1; Long, Xiangyu1; Hu, Songnian2; Tang, Chaorong1
刊名Scientific reports
2016-09-13
卷号6页码:10
ISSN号2045-2322
DOI10.1038/srep33151
通讯作者Hu, songnian(husn@big.ac.cn) ; Tang, chaorong(chaorongtang@126.com)
英文摘要Along with changes in morphology in the course of maturation, leaves of hevea brasiliensis become more resistant to leaf diseases, including the south american leaf blight (salb), a devastating fungal disease of this economically important tree species. to understand the underlying mechanisms of this defense, and to identify the candidate genes involved, we sequenced the hevea leaf transcriptome at four developmental stages (i to iv) by illumina sequencing. a total of 62.6 million high-quality reads were generated, and assembled into 98,796 unique transcripts. we identified 3,905 differentially expressed genes implicated in leaf development, 67.8% (2,651) of which were during the transition to leaf maturation. the genes involved in cyanogenic metabolism, lignin and anthocyanin biosynthesis were noteworthy for their distinct patterns of expression between developing leaves (stages i to iii) and mature leaves (stage iv), and the correlation with the change in resistance to salb and the oidium/colletotrichum leaf fall. the results provide a first profile of the molecular events that relate to the dynamics of leaf morphology and defense strategies during hevea leaf development. this dataset is beneficial to devising strategies to engineer resistance to leaf diseases as well as other in-depth studies in hevea tree.
WOS关键词RNA-SEQ DATA ; MANIHOT-ESCULENTA CRANTZ ; AMERICAN LEAF-BLIGHT ; CYANOGENIC GLUCOSIDES ; FUNCTIONAL ANNOTATION ; HYDROXYNITRILE LYASE ; SUCROSE SYNTHASE ; REFERENCE GENOME ; GENE-EXPRESSION ; WEB SERVER
WOS研究方向Science & Technology - Other Topics
WOS类目Multidisciplinary Sciences
语种英语
出版者NATURE PUBLISHING GROUP
WOS记录号WOS:000382932600001
内容类型期刊论文
URI标识http://www.corc.org.cn/handle/1471x/2376331
专题中国科学院大学
通讯作者Hu, Songnian; Tang, Chaorong
作者单位1.Chinese Acad Trop Agr Sci, Rubber Res Inst, Danzhou 571737, Hainan, Peoples R China
2.Chinese Acad Sci, Beijing Inst Genom, CAS Key Lab Genome Sci & Informat, Beijing 100101, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714
Fang, Yongjun,Mei, Hailiang,Zhou, Binhui,et al. De novo transcriptome analysis reveals distinct defense mechanisms by young and mature leaves of hevea brasiliensis (para rubber tree)[J]. Scientific reports,2016,6:10.
APA Fang, Yongjun.,Mei, Hailiang.,Zhou, Binhui.,Xiao, Xiaohu.,Yang, Meng.,...&Tang, Chaorong.(2016).De novo transcriptome analysis reveals distinct defense mechanisms by young and mature leaves of hevea brasiliensis (para rubber tree).Scientific reports,6,10.
MLA Fang, Yongjun,et al."De novo transcriptome analysis reveals distinct defense mechanisms by young and mature leaves of hevea brasiliensis (para rubber tree)".Scientific reports 6(2016):10.
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