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Mutagenic effects of carbon ion beam irradiations on dry lotus japonicus seeds
Luo, Shanwei1,2; Zhou, Libin1; Li, Wenjian1; Du, Yan1; Yu, Lixia1,2; Feng, Hui1,2; Mu, Jinhu1,2; Chen, Yuze3
刊名Nuclear instruments & methods in physics research section b-beam interactions with materials and atoms
2016-09-15
卷号383页码:123-128
关键词Carbon ion beam Lotus japonicus Mutation breeding Issr
ISSN号0168-583X
DOI10.1016/j.nimb.2016.06.021
通讯作者Zhou, libin(libinzhou@impcas.ac.cn)
英文摘要Carbon ion beam irradiation is a powerful method for creating mutants and has been used in crop breeding more and more. to investigate the effects of carbon ion beams on lotus japonicus, dry seeds were irradiated by 80 mev/u carbon ion beam at dosages of 0, 100, 200, 300, 400, 500 and 600 gy. the germination rate, survival rate and root length of m-1 populations were explored and the dose of 400 gy was selected as the median lethal dose (ld50) for a large-scale mutant screening. among 2472 m-2 plants, 127 morphological mutants including leaf, stem, flower and fruit phenotypic variation were found, and the mutation frequency was approximately 5.14%. inter simple sequence repeat (issr) assays were utilized to investigate the dna polymorphism between seven mutants and eight plants without phenotypic variation from m-2 populations. no remarkable differences were detected between these two groups, and the total polymorphic rate was 0.567%. (c) 2016 elsevier b.v. all rights reserved.
WOS关键词REPEAT ISSR POLYMORPHISM ; ARABIDOPSIS-THALIANA ; GENETIC DIVERSITY ; IONIZING-RADIATION ; MUTATION-RATE ; DNA MARKERS ; HEAVY-IONS ; MUTANTS ; PLANTS ; GERMINATION
WOS研究方向Instruments & Instrumentation ; Nuclear Science & Technology ; Physics
WOS类目Instruments & Instrumentation ; Nuclear Science & Technology ; Physics, Atomic, Molecular & Chemical ; Physics, Nuclear
语种英语
出版者ELSEVIER SCIENCE BV
WOS记录号WOS:000383007300016
内容类型期刊论文
URI标识http://www.corc.org.cn/handle/1471x/2376227
专题中国科学院大学
通讯作者Zhou, Libin
作者单位1.Chinese Acad Sci, Inst Modern Phys, Biophys Grp, 509 Nanchang Rd, Lanzhou 730000, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Gansu Agr Univ, Coll Life Sci & Technol, 1 Yingmen Village, Lanzhou 730070, Gansu, Peoples R China
推荐引用方式
GB/T 7714
Luo, Shanwei,Zhou, Libin,Li, Wenjian,et al. Mutagenic effects of carbon ion beam irradiations on dry lotus japonicus seeds[J]. Nuclear instruments & methods in physics research section b-beam interactions with materials and atoms,2016,383:123-128.
APA Luo, Shanwei.,Zhou, Libin.,Li, Wenjian.,Du, Yan.,Yu, Lixia.,...&Chen, Yuze.(2016).Mutagenic effects of carbon ion beam irradiations on dry lotus japonicus seeds.Nuclear instruments & methods in physics research section b-beam interactions with materials and atoms,383,123-128.
MLA Luo, Shanwei,et al."Mutagenic effects of carbon ion beam irradiations on dry lotus japonicus seeds".Nuclear instruments & methods in physics research section b-beam interactions with materials and atoms 383(2016):123-128.
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