| Furin is involved in baculovirus envelope fusion protein activation | |
| Westenberg, M; Wang, HL; IJkel, WFJ; Goldbach, RW; Vlak, JM; Zuidema, D | |
| 刊名 | Journal of virology
 |
| 2002 | |
| 卷号 | 76期号:1页码:178-184 |
| ISSN号 | 0022-538X |
| DOI | 10.1128/jvi.76.1.178-184.2002 |
| 通讯作者 | Vlak, jm(just.vlak@viro.dpw.wau.nl) |
| 英文摘要 | The spodoptera exigua multicapsid nucleopolyhedrovirus (semnpv) se8 gene was recently shown to encode the viral envelope fusion (f) protein. a 60-kda c-terminal subunit (f(1)) of the 76-kda primary translation product of this gene was found to be the major envelope protein of semnpv budded virus (bv) (w. f. j. ijkel, m. westenberg, r. w. goldbach, g. w. blissard, j. m. vlak, and d. zuidema, virology 275:30-41, 2000). a specific inhibitor was used to show that furin is involved in cleavage of the precursor envelope fusion (f(0)) protein. bv produced in the presence of the inhibitor possesses the uncleaved f(0) protein, while an f protein with a mutation in the furin cleavage site was translocated to the plasma membrane but lost its fusogenic activity. these results indicate that cleavage of f(0) is required to activate the semnpv f protein and is necessary for bv infectivity. specific antibodies against f(1) and against the putative n terminus (f(2)) of the primary translation product were used to show that the f protein is bv specific and that bvs contain both the 60- (f(1)) and 21-kda (f(2)) cleavage products. in nonreducing sodium dodecyl sulfate-polyacrylamide gel electrophoresis both subunits migrate as a single 80-kda protein, indicating that the subunits remain associated by a disulfide linkage. in addition, the presence of the it protein predominately as a monomer suggests that disulfide links are not involved in oligomerization. thus, the envelope fusion protein from group ii nucleopolyhedroviruses of baculoviruses has properties similar to those of proteins from a number of vertebrate viruses. |
| WOS关键词 | NUCLEAR POLYHEDROSIS-VIRUS ; EXIGUA MULTICAPSID NUCLEOPOLYHEDROVIRUS ; SUBTILISIN-LIKE ENDOPROTEASE ; CELL-CELL FUSION ; SPODOPTERA-EXIGUA ; MEMBRANE-FUSION ; LYMANTRIA-DISPAR ; CLEAVAGE SITE ; WILD-TYPE ; GP64 |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| 出版者 | AMER SOC MICROBIOLOGY |
| WOS记录号 | WOS:000172644900018 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2376109 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Vlak, JM |
| 作者单位 | 1.Univ Wageningen & Res Ctr, Virol Lab, NL-6709 PD Wageningen, Netherlands 2.Chinese Acad Sci, Wuhan Inst Virol, Joint Lab Invertebrate Virol, Wuhan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Westenberg, M,Wang, HL,IJkel, WFJ,et al. Furin is involved in baculovirus envelope fusion protein activation[J]. Journal of virology,2002,76(1):178-184. |
| APA | Westenberg, M,Wang, HL,IJkel, WFJ,Goldbach, RW,Vlak, JM,&Zuidema, D.(2002).Furin is involved in baculovirus envelope fusion protein activation.Journal of virology,76(1),178-184. |
| MLA | Westenberg, M,et al."Furin is involved in baculovirus envelope fusion protein activation".Journal of virology 76.1(2002):178-184. |
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