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	Genetic engineering of helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus as an improved pesticide
	Chen, XW; Sun, XL; Hu, ZH; Li, M; O'Reilly, DR; Zuidema, D; Vlak, JM
刊名	Journal of invertebrate pathology
	2000-08-01
卷号	76期号:2页码:140-146
ISSN号	0022-2011
DOI	10.1006/jipa.2000.4963
通讯作者	Vlak, jm()
英文摘要	The helicoverpa armigera single-nucleocapsid nucleopslyhedrovirus (hearnpv) has been registered and is commercially produced in china as a biopesticide to control the bollworm in cotton. however, the virus has a relatively slow speed of action. to improve its efficacy, recombinant hearnpvs were generated by deleting the ecdysteroid udp-glucosyltransferase (egt) gene (hacxw1 and halm2) or by inserting the insect-specific toxin gene aait in the egt locus (hacxw2) of hearnpv using conventional recombination strategies in insect cell culture. the various recombinants remained genetically stable when cultured in hzam1 insect cells. bioassay data showed a significant reduction in the time required for all hearnpv recombinants to kill second instar h. armigera larvae. the lt50 of the egt deletion recombinants hacxw1 and halm2 was about 27% faster than that of wild-type hearnpv. the largest reduction in lt50 was achieved by inserting the gene for the insect-specific neurotoxin, aait, in the egf locus, giving a reduction in lt50 of 32% compared to wild-type hearnpv, the ability to genetically improve the properties of hearnpv as a biopesticide provides a further opportunity to develop this virus into a commercially viable product to control the bollworm in china. (c) 2000 academic press.
WOS关键词	ECDYSTEROID UDP-GLUCOSYLTRANSFERASE ; GREEN FLUORESCENT PROTEIN ; BACULOVIRUS-MEDIATED EXPRESSION ; RECOMBINANT BACULOVIRUSES ; NEUROTOXIN ; HELIOTHIS ; DELETION ; EFFICACY ; VIRUS ; LIQUEFACTION
WOS研究方向	Zoology
WOS类目	Zoology
语种	英语
出版者	ACADEMIC PRESS INC
WOS记录号	WOS:000089301200008
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2376091
专题	武汉病毒研究所
通讯作者	Vlak, JM
作者单位	1.Chinese Acad Sci, Wuhan Inst Virol, Joint Lab Invertebrate Virol, Wuhan 430071, Peoples R China 2.Wageningen Univ, Virol Lab, NL-6709 PD Wageningen, Netherlands 3.Univ London Imperial Coll Sci Technol & Med, Dept Biol, London SW7 2AZ, England
推荐引用方式 GB/T 7714	Chen, XW,Sun, XL,Hu, ZH,et al. Genetic engineering of helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus as an improved pesticide[J]. Journal of invertebrate pathology,2000,76(2):140-146.
APA	Chen, XW.,Sun, XL.,Hu, ZH.,Li, M.,O'Reilly, DR.,...&Vlak, JM.(2000).Genetic engineering of helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus as an improved pesticide.Journal of invertebrate pathology,76(2),140-146.
MLA	Chen, XW,et al."Genetic engineering of helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus as an improved pesticide".Journal of invertebrate pathology 76.2(2000):140-146.

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