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	Selection of a specific peptide from a nona-peptide library for in vitro inhibition of grass carp hemorrhage virus replication
	Wang, B; Ke, LH; Jiang, H; Li, CZ; Tien, P
刊名	Virus research
	2000-04-01
卷号	67期号:2页码:119-125
关键词	Grass carp hemorrhage virus Peptide library Inhibition of replication Kidney cell line
ISSN号	0168-1702
通讯作者	Tien, p()
英文摘要	Grass carp hemorrhage virus (gchv), a member of reoviridae, causes severe hemorrhagic disease of grass carp (ctenopharyngodon idellus) in china. icosahedral virions of gchv were used as to assay the effect of specific peptides for the inhibition of gchv infectivity. a random nona-peptide library displayed on phage fuse5 was constructed, and the expressed peptides were fused onto the amino terminus of the minor coat protein iii. by biopanning, the fused peptides were bound to the biotinylated gchv. phages containing specific peptides bound to gchv were eluted and amplified in escherichia coli k91. three rounds of affinity selection enriched the pool of inhibiting peptides. sixteen clones which inhibited the replication of gchv in a grass carp kidney cell line were selected. the tcid50 of gchv was decreased over 10000 x. six clones having the strongest inhibitory effect shared the same dna sequence, with a deduced amino acid sequence of nh2-leu-trp-val-giy-gly-gly-arg-asn-ala-cooh. a synthesized nona-peptide of identical sequence exhibited similar inhibitory activity towards gchv replication in vitro. (c) 2000 elsevier science b.v. all rights reserved.
WOS关键词	PHAGE DISPLAY ; LIGANDS ; ANTIBODY ; AFFINITY
WOS研究方向	Virology
WOS类目	Virology
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000087640200002
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2376075
专题	武汉病毒研究所
通讯作者	Tien, P
作者单位	1.Chinese Acad Sci, Inst Microbiol, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Peoples R China
推荐引用方式 GB/T 7714	Wang, B,Ke, LH,Jiang, H,et al. Selection of a specific peptide from a nona-peptide library for in vitro inhibition of grass carp hemorrhage virus replication[J]. Virus research,2000,67(2):119-125.
APA	Wang, B,Ke, LH,Jiang, H,Li, CZ,&Tien, P.(2000).Selection of a specific peptide from a nona-peptide library for in vitro inhibition of grass carp hemorrhage virus replication.Virus research,67(2),119-125.
MLA	Wang, B,et al."Selection of a specific peptide from a nona-peptide library for in vitro inhibition of grass carp hemorrhage virus replication".Virus research 67.2(2000):119-125.

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