| A novel virus-encoded nucleocytoplasmic shuttling protein: the ul3 protein of herpes simplex virus type 1 | |
| Zheng, Chunfu; Lin, Fusen; Wang, Shuai; Xing, Junji | |
| 刊名 | Journal of virological methods
 |
| 2011-11-01 | |
| 卷号 | 177期号:2页码:206-210 |
| 关键词 | Hsv-1 Ul3 Nuclear localization signal Nuclear export signal Ran-gtp Chromosomal region maintenance 1 |
| ISSN号 | 0166-0934 |
| DOI | 10.1016/j.jviromet.2011.08.004 |
| 通讯作者 | Zheng, chunfu(zheng.alan@hotmail.com) |
| 英文摘要 | Herpes simplex virus type 1 (hsv-1) ul3 protein is a nuclear protein. in this study, the molecular mechanism of the subcellular localization of ul3 was characterized by fluorescence microscopy in living cells. a nuclear localization signal (nls) and a nuclear export signal (nes) were also identified. ul3 was demonstrated to target to the cytoplasm through the nes via chromosomal region maintenance 1 (crm-1) dependent pathway, and to the nucleus through rangtp-dependent mechanism. heterokaryon assays confirmed that ul3 was capable of shuttling between the nucleus and the cytoplasm. these results demonstrate that the ul3 protein is a novel hsv-1 encoded nucleocytoplasmic shuttling protein. (c) 2011 elsevier b.v. all rights reserved. |
| WOS关键词 | NUCLEAR EXPORT SIGNAL ; OPEN READING FRAME ; LOCALIZATION ; IDENTIFICATION ; TRANSPORT ; THERAPY ; IMPORT ; GENOME ; GENES ; U(L)3 |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Virology |
| WOS类目 | Biochemical Research Methods ; Biotechnology & Applied Microbiology ; Virology |
| 语种 | 英语 |
| 出版者 | ELSEVIER SCIENCE BV |
| WOS记录号 | WOS:000296305400012 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2376018 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Zheng, Chunfu |
| 作者单位 | Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Mol Virol & Viral immunol Res Grp, Wuhan 430071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zheng, Chunfu,Lin, Fusen,Wang, Shuai,et al. A novel virus-encoded nucleocytoplasmic shuttling protein: the ul3 protein of herpes simplex virus type 1[J]. Journal of virological methods,2011,177(2):206-210. |
| APA | Zheng, Chunfu,Lin, Fusen,Wang, Shuai,&Xing, Junji.(2011).A novel virus-encoded nucleocytoplasmic shuttling protein: the ul3 protein of herpes simplex virus type 1.Journal of virological methods,177(2),206-210. |
| MLA | Zheng, Chunfu,et al."A novel virus-encoded nucleocytoplasmic shuttling protein: the ul3 protein of herpes simplex virus type 1".Journal of virological methods 177.2(2011):206-210. |
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