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	Dna probe functionalized qcm biosensor based on gold nanoparticle amplification for bacillus anthracis detection
	Hao, Rong-Zhang2,3; Song, Hong-Bin3; Zuo, Guo-Min2; Yang, Rui-Fu4; Wei, Hong-Ping1; Wang, Dian-Bing1; Cui, Zong-Qiang1; Zhang, ZhiPing1; Cheng, Zhen-Xing2; Zhang, Xian-En1
刊名	Biosensors & bioelectronics
	2011-04-15
卷号	26期号:8页码:3398-3404
关键词	Bacillus anthracis Biosensor Quartz crystal microbalance (qcm) Dna probe Self-assembly Electrochemistry
ISSN号	0956-5663
DOI	10.1016/j.bios.2011.01.010
通讯作者	Zhang, zhiping(zhangzp@wh.iov.cn)
英文摘要	The rapid detection of bacillus anthracis, the causative agent of anthrax disease, has gained much attention since the anthrax spore bioterrorism attacks in the united states in 2001. in this work, a dna probe functionalized quartz crystal microbalance (qcm) biosensor was developed to detect b. anthracis based on the recognition of its specific dna sequences, i.e., the 168 bp fragment of the ba813 gene in chromosomes and the 340 bp fragment of the pag gene in plasmid px01. a thiol dna probe was immobilized onto the qcm gold surface through self-assembly via au-s bond formation to hybridize with the target ss-dna sequence obtained by asymmetric pcr. hybridization between the target dna and the dna probe resulted in an increase in mass and a decrease in the resonance frequency of the qcm biosensor. moreover, to amplify the signal, a thiol-dna fragment complementary to the other end of the target dna was functionalized with gold nanoparticles. the results indicate that the dna probe functionalized qcm biosensor could specifically recognize the target dna fragment of b. anthracis from that of its closest species, such as bacillus thuringiensis, and that the limit of detection (lod) reached 3.5 x 10(2) cfu/ml of b. anthracis vegetative cells just after asymmetric pcr amplification, but without culture enrichment. the dna probe functionalized qcm biosensor demonstrated stable, pollution-free, real-time sensing, and could find application in the rapid detection of b. anthracis. (c) 2011 elsevier b.v. all rights reserved.
WOS关键词	ESCHERICHIA-COLI O157-H7 ; RAPID-DETECTION ; PROTEIN ; SENSORS ; TOXIN ; CHIP ; PCR
WOS研究方向	Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
WOS类目	Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology
语种	英语
出版者	ELSEVIER ADVANCED TECHNOLOGY
WOS记录号	WOS:000289863900002
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2375973
专题	武汉病毒研究所
通讯作者	Zhang, ZhiPing
作者单位	1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 2.Inst Chem Def, Dept 3, Beijing 102205, Peoples R China 3.PLA Inst Dis Control & Prevent, Beijing 100071, Peoples R China 4.Acad Mil Med Sci, Inst Microbiol & Epidemiol, State Key Lab Pathogen & Biosecur, Beijing 100071, Peoples R China
推荐引用方式 GB/T 7714	Hao, Rong-Zhang,Song, Hong-Bin,Zuo, Guo-Min,et al. Dna probe functionalized qcm biosensor based on gold nanoparticle amplification for bacillus anthracis detection[J]. Biosensors & bioelectronics,2011,26(8):3398-3404.
APA	Hao, Rong-Zhang.,Song, Hong-Bin.,Zuo, Guo-Min.,Yang, Rui-Fu.,Wei, Hong-Ping.,...&Zhang, Xian-En.(2011).Dna probe functionalized qcm biosensor based on gold nanoparticle amplification for bacillus anthracis detection.Biosensors & bioelectronics,26(8),3398-3404.
MLA	Hao, Rong-Zhang,et al."Dna probe functionalized qcm biosensor based on gold nanoparticle amplification for bacillus anthracis detection".Biosensors & bioelectronics 26.8(2011):3398-3404.

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