Porous nanosheet-based ZnO microspheres for the construction of direct electrochemical biosensors

	Porous nanosheet-based ZnO microspheres for the construction of direct electrochemical biosensors
	Lu, Xianbo; Zhang, Haijun; Ni, Yuwen; Zhang, Qing; Chen, Jiping
刊名	biosensors & bioelectronics
	2008-09-15
卷号	24 期号:1 页码:93-98
关键词	biosensor direct electron transfer hydrogen peroxide nitrite ZnO
通讯作者	陈吉平
产权排序	1;1
英文摘要	nanosheet-based zno microsphere with porous nanostructures was synthesized by a facile chemical bath deposition method followed by thermal treatment. which was explored for the construction of electrochemical biosensors. spectroscopic and electrochemical researches revealed the zno-based composite was a biocompatible immobilization matrix for enzymes with good enzymatic stability and bioactivity. with advantages of nanostructured inorganic-organic hybrid materials, a pair of stable and well-defined quasi-reversible redox peaks of hemoglobin was obtained with a formal potential of -0.345 v (vs. ag/agcl) in ph 7.0 buffer. facilitated direct electron transfer of the metalloenzymes with an apparent heterogeneous electron transfer rate constant (k(s)) of 3.2 s(-1) was achieved on the zno-based enzyme electrode. comparative studies demonstrated the nanosheet-based zno microspheres were more effective in facilitating the electron transfer of immobilized enzyme than solid zno microspheres, which may result from the unique nanostructures and larger surface area of the porous zno. the prepared biosensor displayed good performance for the detection of h2o2 and nano2 with a wide linear range of 1-410 and 10-2700 mu m, respectively. the entrapped hemoglobin exhibits high peroxidase-like activity for the catalytic reduction of h2o2 with an apparent michaelis-menten constant (k-m(app)) of 143 mu m. the nanosheet-based zno could be a promising matrix for the fabrication of direct electrochemical biosensors, and may find wide potential applications in biomedical detection and environmental analysis. (c) 2008 elsevier b.v. all rights reserved.
WOS标题词	science & technology ; life sciences & biomedicine ; physical sciences
类目[WOS]	biophysics ; biotechnology & applied microbiology ; chemistry, analytical ; electrochemistry ; nanoscience & nanotechnology
研究领域[WOS]	biophysics ; biotechnology & applied microbiology ; chemistry ; electrochemistry ; science & technology - other topics
关键词[WOS]	hydrogen-peroxide biosensor ; direct electron-transfer ; heme-proteins ; carbon nanotube ; zinc-oxide ; hemoglobin ; chitosan ; films ; electrocatalysis ; immobilization
收录类别	SCI
原文出处	true
语种	英语
WOS记录号	WOS:000259425300014
公开日期	2010-11-30
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/100687]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Lu, Xianbo,Zhang, Haijun,Ni, Yuwen,et al. Porous nanosheet-based ZnO microspheres for the construction of direct electrochemical biosensors[J]. biosensors & bioelectronics,2008,24(1):93-98.
APA	Lu, Xianbo,Zhang, Haijun,Ni, Yuwen,Zhang, Qing,&Chen, Jiping.(2008).Porous nanosheet-based ZnO microspheres for the construction of direct electrochemical biosensors.biosensors & bioelectronics,24(1),93-98.
MLA	Lu, Xianbo,et al."Porous nanosheet-based ZnO microspheres for the construction of direct electrochemical biosensors".biosensors & bioelectronics 24.1(2008):93-98.