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	Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus orf51 is a chab homologous gene involved in budded virus production and dna replication
	Zheng, Fangliang1,2; Huang, Yi1; Long, Gang1; Sun, Xiulian1; Wang, Hanzhong1
刊名	Virus research
	2011
卷号	155期号:1页码:203-212
关键词	Baculovirus Hearnpv orf51 (ha51) Chab Bv production Dna replication
ISSN号	0168-1702
DOI	10.1016/j.virusres.2010.10.006
通讯作者	Huang, yi(hy@wh.iov.cn)
英文摘要	The baculovirus chab proteins are conserved in all completely sequenced lepidopteran npvs and are annotated as putative dna binding proteins. here we investigated helicoverpa annigera single nucleocapsid nucleopolyhedrovirus (hearnpv) orf51 (ha51). one of the chab homologues in hearnpv. 5`-race revealed that ha51 is transcribed from a conventional early promoter transcriptional initiator motif (catt) located at 159nt upstream of atg. rt-pcr confirmed that ha51 is an early transcribed gene. to study the function of ha51 in the life cycle of hearnpv, ha51 knockout and repair bacmids were generated by homologous recombination in escherichia coli. growth curve and dna replication analyses showed that the levels of budded virus (bv) production and viral dna accumulation were significantly higher in cells infected with ha51 null virus than those infected with wild-type bacmid derived virus. electron microscopy revealed that polyhedra formation was not affected by the deletion of ha51. bioassay demonstrated that the ha51-deleted virus had similar oral infectivity as the wild-type and rescued virus. western blot analyses suggested that ha51 is a component of the nucleocapsid of bv and occlusion-derived virus as well as the envelope of bv. immunofluorescence microscopy showed that ha51 protein is mainly localized in the cytoplasm of infected cells. taken together, our results indicate that, unlike previously characterized baculovirual chab genes, ha51 is involved in viral dna replication and bv production and is transcribed in the early stage of infection. (c) 2010 elsevier b.v. all rights reserved.
WOS关键词	CELL-CYCLE ARREST ; AUTOGRAPHA-CALIFORNICA NUCLEOPOLYHEDROVIRUS ; ESCHERICHIA-COLI ; MULTICAPSID NUCLEOPOLYHEDROVIRUS ; ACCUMULATION ; PROTEINS ; SEQUENCE ; GENOMES ; CLONING ; PHASE
WOS研究方向	Virology
WOS类目	Virology
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000287010300028
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2375881
专题	武汉病毒研究所
通讯作者	Huang, Yi
作者单位	1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Zheng, Fangliang,Huang, Yi,Long, Gang,et al. Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus orf51 is a chab homologous gene involved in budded virus production and dna replication[J]. Virus research,2011,155(1):203-212.
APA	Zheng, Fangliang,Huang, Yi,Long, Gang,Sun, Xiulian,&Wang, Hanzhong.(2011).Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus orf51 is a chab homologous gene involved in budded virus production and dna replication.Virus research,155(1),203-212.
MLA	Zheng, Fangliang,et al."Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus orf51 is a chab homologous gene involved in budded virus production and dna replication".Virus research 155.1(2011):203-212.

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