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	Production and characterization of monoclonal antibodies against the hemagglutinin of h5n1 and antigenic investigation of avian influenza h5n1 viruses isolated from china
	Wei, Jie; Yan, Bing; Chen, Ze; Li, Tianxian; Deng, Fei; Wang, Hualin; Hu, Zhihong
刊名	Canadian journal of microbiology
	2011
卷号	57期号:1页码:42-48
关键词	Antigenic characterization Epitope H5n1 Monoclonal antibodies Phylogeny
ISSN号	0008-4166
DOI	10.1139/w10-097
通讯作者	Hu, zhihong(huzh@wh.iov.cn)
英文摘要	Eight monoclonal antibodies against hemagglutinin of influenza a virus a/chicken/henan/01/2004(h5n1) were produced by a dna prime and inactivated virions-boost immunization strategy. among the monoclonal antibodies, 3 (h50, h56, and h57) exhibited hemagglutination inhibition activity. western blot analyses revealed that all the monoclonal antibodies reacted to the prokaryotically expressed ha1 of a/chicken/henan/01/2004(h5n1). the monoclonal antibodies were then used to characterize 10 avian influenza h5n1 viruses isolated from china during 2004 to 2007, by using the hemagglutination inhibition test and the antigen-capture enzyme-linked immunosorbent assay. the isolates could be divided into 4 different antigenic groups according to their responses to the monoclonal antibodies. the antigenic grouping of these 10 h5n1 isolates, using these antibodies, did not completely match their phylogenetic classification based on the hemagglutinin sequences. the results showed there were antigenic variations within the subclade 2.3.4 of h5n1, which is predominant in china.
WOS关键词	HA DNA VACCINE ; A VIRUS ; IMMUNIZATION ; PROTEIN ; PROTECTION ; HUMANS ; BIRDS ; ASIA
WOS研究方向	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Immunology ; Microbiology
WOS类目	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Immunology ; Microbiology
语种	英语
出版者	CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS
WOS记录号	WOS:000286474800005
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2375875
专题	武汉病毒研究所
通讯作者	Hu, Zhihong
作者单位	Chinese Acad Sci, State Key Lab Virol, Wuhan Inst Virol, Wuhan 430071, Peoples R China
推荐引用方式 GB/T 7714	Wei, Jie,Yan, Bing,Chen, Ze,et al. Production and characterization of monoclonal antibodies against the hemagglutinin of h5n1 and antigenic investigation of avian influenza h5n1 viruses isolated from china[J]. Canadian journal of microbiology,2011,57(1):42-48.
APA	Wei, Jie.,Yan, Bing.,Chen, Ze.,Li, Tianxian.,Deng, Fei.,...&Hu, Zhihong.(2011).Production and characterization of monoclonal antibodies against the hemagglutinin of h5n1 and antigenic investigation of avian influenza h5n1 viruses isolated from china.Canadian journal of microbiology,57(1),42-48.
MLA	Wei, Jie,et al."Production and characterization of monoclonal antibodies against the hemagglutinin of h5n1 and antigenic investigation of avian influenza h5n1 viruses isolated from china".Canadian journal of microbiology 57.1(2011):42-48.

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