| Conserved residues in the aromatic acid/h+ symporter family are important for benzoate uptake by ncgl2325 in corynebacterium glutamicum | |
| Wang, Song-He1; Xu, Ying1; Liu, Shuang-Jiang2; Zhou, Ning-Yi1 | |
| 刊名 | International biodeterioration & biodegradation
 |
| 2011-06-01 | |
| 卷号 | 65期号:3页码:527-532 |
| 关键词 | Benzoate Corynebacterium glutamicum Ncgl2325 Site-directed mutagenesis Transporter |
| ISSN号 | 0964-8305 |
| DOI | 10.1016/j.ibiod.2011.02.004 |
| 通讯作者 | Zhou, ning-yi(n.zhou@pentium.whiov.ac.cn) |
| 英文摘要 | Corynebacterium glutamicum is a model organism for genetic and physiological studies in gram-positive bacteria. ncgl2325 in c. glutamicum, a transporter belonging to the aromatic acid/h+ symporter family, has previously been reported to be involved in benzoate assimilation. here, we showed that this transporter, fused with gfp, was associated with the cell membrane in escherichia coli and c. glutamicum. uptake assays with (c-14]-labeled benzoate demonstrated that ncgl2325 transported benzoate into the cells at a v-max of 0.19 +/- 0.01 nmol/min/mg of dry weight, and the k-m value was determined to be 1.11 +/- 0.24 mu m. among the competing substrates tested, hydroxyl-substituted benzoates resulted in significant inhibition (>50%) of benzoate uptake. site-directed mutagenesis of conserved residues in the hydrophilic cytoplasmic loops (gly-80, asp-84 and asp-312) and the hydrophobic transmembrane regions (asp-35, arg-119, glu-139 and arg-386) resulted in loss of benzoate transport activity. this is the first study to investigate the molecular basis of benzoate transport. (c) 2011 elsevier ltd. all rights reserved. |
| WOS关键词 | METAL-TETRACYCLINE/H+ ANTIPORTER ; MAJOR FACILITATOR SUPERFAMILY ; INNER-MEMBRANE-PROTEINS ; ESCHERICHIA-COLI ; LACTOSE PERMEASE ; PSEUDOMONAS-PUTIDA ; TRANSPOSON TN10 ; AMINO-ACIDS ; TOPOLOGY ; GENOME |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Environmental Sciences & Ecology |
| WOS类目 | Biotechnology & Applied Microbiology ; Environmental Sciences |
| 语种 | 英语 |
| 出版者 | ELSEVIER SCI LTD |
| WOS记录号 | WOS:000290693900021 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2375859 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Zhou, Ning-Yi |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, Key Lab Agr & Environm Microbiol, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, State Key Lab Microbial Resources, Inst Microbiol, Beijing 100101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Song-He,Xu, Ying,Liu, Shuang-Jiang,et al. Conserved residues in the aromatic acid/h+ symporter family are important for benzoate uptake by ncgl2325 in corynebacterium glutamicum[J]. International biodeterioration & biodegradation,2011,65(3):527-532. |
| APA | Wang, Song-He,Xu, Ying,Liu, Shuang-Jiang,&Zhou, Ning-Yi.(2011).Conserved residues in the aromatic acid/h+ symporter family are important for benzoate uptake by ncgl2325 in corynebacterium glutamicum.International biodeterioration & biodegradation,65(3),527-532. |
| MLA | Wang, Song-He,et al."Conserved residues in the aromatic acid/h+ symporter family are important for benzoate uptake by ncgl2325 in corynebacterium glutamicum".International biodeterioration & biodegradation 65.3(2011):527-532. |
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