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	Cell aggregation induces phosphorylation of pecam-1 and pyk2 and promotes tumor cell anchorage-independent growth
	Zhang, Xing2; Xu, Li-hua2; Yu, Qiang1
刊名	Molecular cancer
	2010-01-14
卷号	9页码:11
ISSN号	1476-4598
DOI	10.1186/1476-4598-9-7
通讯作者	Yu, qiang(qyu@sibs.ac.cn)
英文摘要	Background: apoptosis caused by inadequate or inappropriate cell-matrix interactions is defined as anoikis. although transformed cells are known to be anoikis-resistant, the underlying mechanisms have not been well understood. we investigated the mechanisms of anoikis resistance of tumor cells. results: we observed that cell aggregation in suspension promoted cell survival and proliferation. we demonstrated a correlation between tumor cell aggregation in suspension and cell growth in soft agar. analysis of tyrosine kinase-mediated cell survival and growth signaling pathways revealed increased levels of tyrosine-phosphorylation of pecam-1 and pyk2 in cell aggregates. we also showed that pecam-1 and pyk2 physically interact with each other, and that pecam-1 carrying a deletion of exons 11-16 could no longer bind to pyk2. furthermore, rna interference-mediated reduction of pyk2 and pecam-1 protein levels reduced cell aggregation and inhibited the growth of tumor cells in soft agar. conclusions: the data demonstrated that pyk2 and pecam-1 were critical mediators of both anchorage-independent growth and anoikis resistance in tumor cells.
WOS关键词	FOCAL ADHESION KINASE ; LUNG ADENOCARCINOMA CELLS ; ACTIVATED PROTEIN-KINASE ; QUANTITATIVE IMMUNOCYTOCHEMICAL ASSAYS ; BREAST-CANCER CELLS ; TYROSINE KINASE ; E-CADHERIN ; EPITHELIAL-CELLS ; TRANSFORMED-CELLS ; SIGNALING PATHWAY
WOS研究方向	Biochemistry & Molecular Biology ; Oncology
WOS类目	Biochemistry & Molecular Biology ; Oncology
语种	英语
出版者	BIOMED CENTRAL LTD
WOS记录号	WOS:000274836800001
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2375833
专题	武汉病毒研究所
通讯作者	Yu, Qiang
作者单位	1.Chinese Acad Sci, Shanghai Inst Mat Med, Dept Pharmacol, Shanghai 201203, Peoples R China 2.Sun Yat Sen Univ, Ctr Canc, State Key Lab Oncol So China, Guangzhou 510060, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Xing,Xu, Li-hua,Yu, Qiang. Cell aggregation induces phosphorylation of pecam-1 and pyk2 and promotes tumor cell anchorage-independent growth[J]. Molecular cancer,2010,9:11.
APA	Zhang, Xing,Xu, Li-hua,&Yu, Qiang.(2010).Cell aggregation induces phosphorylation of pecam-1 and pyk2 and promotes tumor cell anchorage-independent growth.Molecular cancer,9,11.
MLA	Zhang, Xing,et al."Cell aggregation induces phosphorylation of pecam-1 and pyk2 and promotes tumor cell anchorage-independent growth".Molecular cancer 9(2010):11.

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